1.13.12.16: nitronate monooxygenase

This is an abbreviated version!
For detailed information about nitronate monooxygenase, go to the full flat file.

Reaction

Synonyms

2-nitropropane dioxygenase, EC 1.13.11.32, NAO, ncd2, nitroalkane oxidase, nitroalkane-oxidizing enzyme, nitronate monooxygenase, nitropropane dioxygenase, NMO, NMO-Nc, NMO-Ws, nmoA, Npd1, Npd2, Npd3, Npd4, Npd5, Npd6, oxidase, nitroalkane, oxygenase, 2-nitropropane di-, P3N monooxygenase, Pa-NMO, PA4202, protein PA4202, Rv1894c, Sa-NAO

ECTree

     1 Oxidoreductases

         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)

                1.13.12.16 nitronate monooxygenase

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Results	in table
3	Activating Compound
3508	AA Sequence
2	CAS Registry Number
13	Cloned(Commentary)
32	Cofactor
5	Crystallization (Commentary)
3	Expression
8	General Information
3	General Stability
84	Inhibitors
57	kcat/KM [mM/s]
20	Ki Value [mM]
122	KM Value [mM]
9	Metals/Ions
16	Molecular Weight [Da]
20	Natural Substrates/ Products (Substrates)
57	Organism
3	Pathway
16	PDB ID
26	pH Optimum
5	pH Range
1	pH Stability
11	Protein Variants
21	Purification (Commentary)
11	Reaction
3	Reaction Type
40	Reference
4	Source Tissue
9	Specific Activity [micromol/min/mg]
2	Storage Stability
234	Substrates and Products (Substrate)
14	Subunits
76	Synonyms
1	Systematic Name
21	Temperature Optimum [°C]
3	Temperature Range [°C]
6	Temperature Stability [°C]
67	Turnover Number [1/s]

Crystallization

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Crystallization on EC 1.13.12.16 - nitronate monooxygenase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

crystallization of the substrate and the complex

Cyberlindnera mrakii

-

698266

hanging drop vapor diffusion method, using 21% (w/v) PEG 3350, 70 mM sodium citrate at pH 4.0, and 100 mM ammonium acetate

Cyberlindnera saturnus

-

765734

hanging drop vapour diffusion method using 10% (w/v) polyethylene glycol monomethyl ether 5000

Pseudomonas aeruginosa

Q9I4V0

674590

purified recombinant His-tagged enzyme, vapor diffusion hanging drop method at room temperature, 0.002 ml of 14 mg/ml protein in 50 mM Tris-Cl, pH 8.0, 100 mM NaCl, 20 mM 2-nitropropane, with 0.002 ml of reservoir solution containing 14% w/v PEG 5000 monomethylether and 0.1 M Na-HEPES, pH 7.0, and equilibration against 1 ml of reservoir solution, 7-10 days, X-ray diffraction structure determination and analysis at 1.44 A resolution, molecular replacement

Pseudomonas aeruginosa

Q9HWH9

745306

crystal structure of NAO from Streptomyces ansochromogenes is determined. It consists of two domains, a TIM barrel domain bound to FMN and C-terminal domain with a novel folding pattern

Streptomyces ansochromogenes

Q9FDD4

724175