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1.13.12.17: dichloroarcyriaflavin A synthase

This is an abbreviated version!
For detailed information about dichloroarcyriaflavin A synthase, go to the full flat file.

Reaction

dichlorochromopyrrolate
+ 4 O2 + 4 NADH + 4 H+ =
dichloroarcyriaflavin A
+ 2 CO2 + 6 H2O + 4 NAD+

Synonyms

CYP245A1, cytochrome P450 StaP, P450 StaP, StaP

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
                1.13.12.17 dichloroarcyriaflavin A synthase

Cloned

Cloned on EC 1.13.12.17 - dichloroarcyriaflavin A synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of StaP, StaC, and RebC in Escherichia coli
the coding region for the enzyme component StaP is cloned into expression vector pET26b(+) (Novagen, San Diego, CA), with a C-terminal 6 * His tag, and overexpressed in Escherichia coli strain BL21