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1.13.12.18: dinoflagellate luciferase

This is an abbreviated version!
For detailed information about dinoflagellate luciferase, go to the full flat file.

Word Map on EC 1.13.12.18

Reaction

dinoflagellate luciferin
+
O2
=
oxidized dinoflagellate luciferin
+
H2O
+
hnu

Synonyms

D2-LCF, D3-LCF, LCF, luciferase

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
                1.13.12.18 dinoflagellate luciferase

Purification

Purification on EC 1.13.12.18 - dinoflagellate luciferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation
-
ammonium sulfate precipitation and DEAE-Bio-Gel A column chromatography
-
ammonium sulfate precipitation, Chelex column chromatography, hydroxylapatite column chromatography, and Ultrogel gel filtration
DEAE column chromatography
-
domain D2-LCF and D3-LCF
-
glutathione-Sepharose column chromatography
-
Ni-NTA column chromatography
-
Ni-NTA resin column chromatography
-
nickel metal chelate column chromatography