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1.13.12.5: Renilla-type luciferase

This is an abbreviated version!
For detailed information about Renilla-type luciferase, go to the full flat file.

Word Map on EC 1.13.12.5

Reaction

coelenterazine h
+
O2
=
coelenterazine h dioxetanone

Synonyms

19kOLase, aequorin, aequorin-1, BFP-aq, blue fluorescent protein from the calcium-binding photoprotein aequorin, Caussia princeps luciferase, clytin, extGLuc, extRLuc, firefly luciferase, fluc, Gaussia luciferase, Gaussia luciferase enzyme, Gaussia princeps luciferase, Gaussia-luciferase, GFP-aq, GLase, GLuc, Luc, luciferase, luciferase (Renilla luciferin), m-Rluc8, membrane-anchored RLUc, Oplophorus luciferase, PI-Rluc, R-Luc, Renilla luciferase, Renilla luciferase-547, Renilla luciferin 2-monooxygenase, Renilla muelleri luciferase, Renilla reniformis luciferase, Renilla-luciferase, Renilla-luciferin 2-monooxygenase, Renilla-type luciferase, RLase, RLase-547, RLuc, RLuc8, short peptide-inserted-Renilla luciferase

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
                1.13.12.5 Renilla-type luciferase

Cloned

Cloned on EC 1.13.12.5 - Renilla-type luciferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
catalytic domain (19 kDa) is expressed using a cold-induced expression system in Escherichia coli as His-tagged fusion proteins
-
expressed as a soluble form in Escherichia coli BL21(DE3)pLysS (Novagen) containing the expression vector pET-21a inserted with cDNA of Renilla luciferase
CAA01908.1
expressed in Escherichia coli
-
expressed in HEK-293T cells
Renilla sp.
-
expressed using a cold-induced expression system in Escherichia coli as His-tagged fusion proteins
expression in 293T HEK fibroblasts and HF8 human fibroblasts
-
expression in Arabidopsis
-
expression in Chlamydomonas reinhardtii
expression in CHO cells and HepG2 cells
expression in CHO-K1 cells and PC12D cells
-
expression in Escherichia coli
expression in Escherichia coli BL21
-
expression in Escherichia coli BL21 Star (DE3)
expression in Escherichia coli DH10B cells and 293T human kidney fibroblasts
-
expression in Escherichia coli DH5alpha
-
expression in Escherichia coli strain BL21
-
expression in Escherichia coli strain DH5alpha and MCF-7 cells
Renilla sp.
-
expression in Escherichia coli strain ER 2566
Renilla sp.
-
expression in HEK-293 cells or Grip-Tite 293 MSR cells
-
expression in HeLa-cell
-
expression in Hep-G2 cell
Renilla sp.
-
expression in insect cells using a baculovirus expression system
expression in LNCaP cell, pRL-SV40 cell, pRL-null cell
-
expression in mouse and human C57/BL6 primary T cells, expression in PG-13 cells
expression in rabbit leukocyte lysate
Renilla sp.
-
expression of His-tagged apoclytin into the periplasmic space in Escherichia coli
-
expression of Renilla luciferase split fragments in HeLa cells, mitochondrial localization, overview
-
expression of RLuc8 in Escherichia coli BL21
Renilla sp.
-
expression of stabilized variant RLuc8 with noncleavable N-terminal His6-tag
fragments of Renilla luciferase (Rluc) are fused to the chemotaxis-associated response regulator CheY3 and its phosphatase CheZ in the enteric pathogen Vibrio cholerae in order to demonstrate dynamic protein-protein interactions by luciferase complementation
-
gene luc, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
recombinant enzyme expression in ES-2 cells
recombinant expression of engineered His-tagged enzyme C-SRLuc8 in Escherichia coli, method optimization
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 Star (DE3)
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
recombinant expression of soluble His6-tagged enzyme FcUni-RLuc in Escherichia coli strain BL21 (DE3), an Fc-binding peptide is separated by a five-amino acid linker from RLuc