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1.13.12.7: firefly luciferase

This is an abbreviated version!
For detailed information about firefly luciferase, go to the full flat file.

Word Map on EC 1.13.12.7

Reaction

D-firefly luciferin
+
O2
+
ATP
=
firefly oxyluciferin
+
CO2
+
AMP
+
diphosphate
+
hnu

Synonyms

AL1, AL2, beetle luciferase, CBG99luc, CBRluc, FFL, firefly luciferase, firefly luciferin luciferase, fluc, LpLuc1, LpLuc2, Luc, Luc1, Luc1-type luciferase, Luc2, Luc2-type luciferase, luciferase, luciferase (firefly luciferin), luciferase FM, luciferin, Luciola italica luciferase, lucPpe, lucPpy, orange light-producing luciferase, oxygen 4-oxidoreductase, PC3-Luc, Photinus luciferin 4-monooxygenase (ATP-hydrolyzing), Photinus pyralis luciferase, PML, PpLase, Ppy, Ppy GR-TS, Ppy RE-TS, PpyWT, PsntWT

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
                1.13.12.7 firefly luciferase

General Stability

General Stability on EC 1.13.12.7 - firefly luciferase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
activity loss at 42°C is reverted at 4°C
-
bovine serum albumin stabilizes
-
decrease of remaining activity after trypsin hydrolysis at 23 and 37°C, mutants R213M and R337Q are more stable than the wild-type luciferase
-
dithiothreitol stabilizes
-
EDTA stabilizes
-
EDTA, 2-mercaptoethanol, dithiothreitol and bovine serum albumin used as stabilizers
-
enzyme is stable for several days adsorbed on polystyrene
-
immobilization on hexyl-Sepharose reduces the specific activity to 35%, immobilization on octyl-Sepharose reduces the specific activity to 26%
inactivation inhibited by sodium azide
-
more stable in phosphate buffer than in Tricine buffer
-
mutant R337Q still 26% remaining activity after 15 min trypsin hydrolysis at 37°C
-
sorbitol, proline and sucrose with strong stabilizing effect on firefly luciferase activity at 35°C
-
stability in crude solutions varies
-
stability increases 1000fold with immobilization on CNBr-Sepharose 4B, pH 7.5 at 25°C
-
stabilized by immobilization on polysaccharide carriers