1.14.11.11: hyoscyamine (6S)-dioxygenase
This is an abbreviated version!
For detailed information about hyoscyamine (6S)-dioxygenase, go to the full flat file.
Word Map on EC 1.14.11.11
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1.14.11.11
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scopolamine
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tropane
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hyoscyamus
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niger
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belladonna
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atropa
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anisodus
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anticholinergic
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hairy
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acutangulus
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anisodamine
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epoxidation
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2-oxoglutarate-dependent
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aerial
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6beta-hydroxylase
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solanaceous
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tanguticus
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agrobacterium-mediated
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metel
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putrescine
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datura
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n-methyltransferase
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tabacum
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chemotype
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high-value
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catharanthus
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pericycle
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roseus
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tropinone
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genetically-engineered
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biofunctional
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gst-tag
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vindoline
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underground
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bolt
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2.1.1.53
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drug development
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pharmacology
- 1.14.11.11
- scopolamine
-
tropane
- hyoscyamus
- niger
- belladonna
-
atropa
-
anisodus
-
anticholinergic
-
hairy
- acutangulus
-
anisodamine
-
epoxidation
-
2-oxoglutarate-dependent
-
aerial
-
6beta-hydroxylase
-
solanaceous
- tanguticus
-
agrobacterium-mediated
- metel
- putrescine
- datura
- n-methyltransferase
- tabacum
-
chemotype
-
high-value
-
catharanthus
- pericycle
- roseus
- tropinone
-
genetically-engineered
-
biofunctional
-
gst-tag
- vindoline
-
underground
-
bolt
-
2.1.1.53
- drug development
- pharmacology
Reaction
Synonyms
6H6, AaH6H, AtH6H, Dih6h, H6H, HnH6H, hyoscyamine 6-hydroxylase, hyoscyamine 6beta-hydroxylase, hyoscyamine-6beta-hydroxylase, oxygenase, hyoscyamine 6beta-di-
ECTree
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Engineering
Engineering on EC 1.14.11.11 - hyoscyamine (6S)-dioxygenase
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K97A
the shows a 3.3fold improved hydroxylation activity compared with the wild-type enzyme
K97E
the shows a 2.7fold improved hydroxylation activity compared with the wild-type enzyme
K97G
the shows a 1.7fold improved hydroxylation activity compared with the wild-type enzyme
S14H
the shows a 2.4fold improved hydroxylation activity compared with the wild-type enzyme
S14P
the shows a 3.7fold improved hydroxylation activity compared with the wild-type enzyme
S14P/K97A
double mutant shows a 4.4fold improved hydroxylation activity compared with the wild-type enzyme, and the in vivo epoxidation activity is also improved by 2.3times. With this mutant, in a 5 l bioreactor with a working volume of 3 l, scopolamine is produced via a single-enzyme-mediated two-step transformation from hyoscyamine of 500 mg/l in 97% yield (1.068 g)
additional information
enhanced production of tropane alkaloids in transgenic Anisodus acutangulus hairy root cultures by overexpressing tropinone reductase I and hyoscyamine-6beta-hydroxylase Agrobacterium-mediated gene transfer technology, quantification of tropan alkaloid contents in transgenic lines, overview
additional information
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enhanced production of tropane alkaloids in transgenic Anisodus acutangulus hairy root cultures by overexpressing tropinone reductase I and hyoscyamine-6beta-hydroxylase Agrobacterium-mediated gene transfer technology, quantification of tropan alkaloid contents in transgenic lines, overview
additional information
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truncation of an H6H enzyme flexible N-terminal region yields an active and stable yet more compact enzyme version
additional information
functional expression in Saccharomyces cerevisiae as tagged and untagged proteins. The untagged form is about 3-fold as active as the tagged protein