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A141E
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mutation enhances grain yield, mutation enhances disease resistance
E140A
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mutation enhances grain yield, mutation enhances disease resistance
G434A
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mutation enhances disease resistance
additional information
a loss-of-function mutation of isoform ga2ox2 causes an increase in gibberellin 4 levels and partly suppresses the germination inability during dark imbibition after inactivation of phytochrome. The efficiency of deactivation of exogenous gibberellin 4 by the enzyme is dependent on light conditions
additional information
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a loss-of-function mutation of isoform ga2ox2 causes an increase in gibberellin 4 levels and partly suppresses the germination inability during dark imbibition after inactivation of phytochrome. The efficiency of deactivation of exogenous gibberellin 4 by the enzyme is dependent on light conditions
additional information
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scc7-D is an allele of isoform GA2ox8 and a genetic suppressor of the long-hypocotyl phenotype of the cryptochrome1/cryptochrome2 mutation in a light-dependent manner. Cryptochromes are required for the blue light regulation of GA2ox1, GA20ox1, and GA3ox1 expression in transient induction, continuous illumination, and photoperiodic conditions
additional information
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loss-of-funtion mutation of GA2ox7 suppresses the dwarf phenotype of DWARF AND DELAYED FLOWERING 1 (DDF1) mutants, indicating that their gibberellin deficiency is due to overexpression of GA2ox7, DDF1 activates the promoter of GA2ox7 in leaves. Under high-salinity stress (250 mM), osmotic stress (300 mM mannitol) GA2ox7 is unregulated and slightly unregulated under the stress hormone abscisic acid (100 microM), the GA2ox7 loss-of-function mutant is less growth retarded than the wild-type under high-salinity stress
additional information
single loss-of-function mutants do not exhibit any obvious phenotype, quintuple mutant (loss of function alleles of all five C19-gibberellin 2-oxidase genes) leads to a complete loss of C19-gibberellin 2-oxidase (C19-GA2ox) activity, with effects on other gibberellin regulated genes (reduced expression of gibberellin biosynthetic genes) and positive regulation of the C20-GA2ox gene GA20x8, like the positive regulation of 4 of the 5 C19-GA2ox genes with the exception of GA2ox3, thus, partial compensation for the activity loss of C19-GA2ox is reached by a combination of feedback and feed-forward regulation of various steps in the gibberellin biosynthesis pathway
additional information
single loss-of-function mutants do not exhibit any obvious phenotype, quintuple mutant (loss of function alleles of all five C19-gibberellin 2-oxidase genes) leads to a complete loss of C19-gibberellin 2-oxidase (C19-GA2ox) activity, with effects on other gibberellin regulated genes (reduced expression of gibberellin biosynthetic genes) and positive regulation of the C20-GA2ox gene GA20x8, like the positive regulation of 4 of the 5 C19-GA2ox genes with the exception of GA2ox3, thus, partial compensation for the activity loss of C19-GA2ox is reached by a combination of feedback and feed-forward regulation of various steps in the gibberellin biosynthesis pathway
additional information
single loss-of-function mutants do not exhibit any obvious phenotype, quintuple mutant (loss of function alleles of all five C19-gibberellin 2-oxidase genes) leads to a complete loss of C19-gibberellin 2-oxidase (C19-GA2ox) activity, with effects on other gibberellin regulated genes (reduced expression of gibberellin biosynthetic genes) and positive regulation of the C20-GA2ox gene GA20x8, like the positive regulation of 4 of the 5 C19-GA2ox genes with the exception of GA2ox3, thus, partial compensation for the activity loss of C19-GA2ox is reached by a combination of feedback and feed-forward regulation of various steps in the gibberellin biosynthesis pathway
additional information
single loss-of-function mutants do not exhibit any obvious phenotype, quintuple mutant (loss of function alleles of all five C19-gibberellin 2-oxidase genes) leads to a complete loss of C19-gibberellin 2-oxidase (C19-GA2ox) activity, with effects on other gibberellin regulated genes (reduced expression of gibberellin biosynthetic genes) and positive regulation of the C20-GA2ox gene GA20x8, like the positive regulation of 4 of the 5 C19-GA2ox genes with the exception of GA2ox3, thus, partial compensation for the activity loss of C19-GA2ox is reached by a combination of feedback and feed-forward regulation of various steps in the gibberellin biosynthesis pathway
additional information
single loss-of-function mutants do not exhibit any obvious phenotype, quintuple mutant (loss of function alleles of all five C19-gibberellin 2-oxidase genes) leads to a complete loss of C19-gibberellin 2-oxidase (C19-GA2ox) activity, with effects on other gibberellin regulated genes (reduced expression of gibberellin biosynthetic genes) and positive regulation of the C20-GA2ox gene GA20x8, like the positive regulation of 4 of the 5 C19-GA2ox genes with the exception of GA2ox3, thus, partial compensation for the activity loss of C19-GA2ox is reached by a combination of feedback and feed-forward regulation of various steps in the gibberellin biosynthesis pathway
additional information
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fusion enzyme MdGA2ox1-MBP
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mutants overexpressing GA2ox9ACT, GA2ox5delta335-341ACT, and GA2ox6, motif III of the conserved motifs characteristic for C20 GA2oxs is necessary for C20 GA2ox activity, motif III is present in rice GA2ox5, GA2ox6, and GAsox9
additional information
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isolation of a CaMV 35S-enhancer activation tagged mutant, H032, showing an increased level of OsGA2ox6 mRNA compared to the wild-type plants. RNAi and ectopic expression analysis of OsGA2ox6 show that the dwarf trait and the decreased levels of bioactive GAs in the H032 mutant are a result of the upregulation of the OsGA2ox6 gene. The H032 mutant retains normal flowering and seed production
additional information
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ectopic expression of gibberllin 2-oxidase in wheat decreases the content of bioactive gibberellins and produces a range of dwarf plants with different degrees of severity. The dwarf phenotype is stably inherited over at least four generations and includes dark-green leaves, increasing tillering and, in severe cases, a prostrate growth habit. Expression of gibberlic acid biosynthesis genens TaGA20ox1 and TaGA3ox2 is up-regulated ant that of two alpha-amylase genes down-regulated in scutella of semi-dwarf lines. The phenotypes are restored to normal by application of gibberellin 3
additional information
expression of isoform PcGA2ox1 in Solanum melanocerasum and Solanum nigrum results in transgenic plants with a range of dwarf phenotypes associated with a severe reduction in the concentrations of biologically active gibberellins 1 and 4. Flowering and fruit development are unaffected. Transgenic plants contain greater concentrations of chlorophyll b and total chlorophyll, although chlorophyll a and carotenoid contents are reduced
additional information
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expression of isoform PcGA2ox1 in Solanum melanocerasum and Solanum nigrum results in transgenic plants with a range of dwarf phenotypes associated with a severe reduction in the concentrations of biologically active gibberellins 1 and 4. Flowering and fruit development are unaffected. Transgenic plants contain greater concentrations of chlorophyll b and total chlorophyll, although chlorophyll a and carotenoid contents are reduced
additional information
isoform GA2ox1 overexpressing transformants exhibit a dwarf phenotype, reduced stolon growth and earlier in vitro tuberization. Transgenic plants with reduced expression of GA2ox1 show normal growth, an altered stolon swelling phenotype, and delayind in vitro tuberization. tubers of Ga2ox1 suppression clones contain increased levels of gibberllin 20
additional information
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isoform GA2ox1 overexpressing transformants exhibit a dwarf phenotype, reduced stolon growth and earlier in vitro tuberization. Transgenic plants with reduced expression of GA2ox1 show normal growth, an altered stolon swelling phenotype, and delayind in vitro tuberization. tubers of Ga2ox1 suppression clones contain increased levels of gibberllin 20