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1.14.12.11: toluene dioxygenase

This is an abbreviated version!
For detailed information about toluene dioxygenase, go to the full flat file.

Word Map on EC 1.14.12.11

Reaction

Toluene
+
NADH
 +
H+
 +
O2
=
(1S,2R)-3-Methylcyclohexa-3,5-diene-1,2-diol
 +
NAD+

Synonyms

ISPTOD, ISPTOL, More, oxygenase, toluene 2,3-di-, oxygenaseTOL, TDO, Tod, todC1C2BA, toluene 2,3-dioxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
                1.14.12.11 toluene dioxygenase

Cloned

Cloned on EC 1.14.12.11 - toluene dioxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned and expressed in Escherichia coli HB101
-
co-overexpression of TDO-F, TDO-R, TDO-O and apo-TDO-O in Escherichia coli
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli DH5alpha cells
-
expressed in Escherichia coli DHalpha
-
expressed in Escherichia coli JM109
-
expressed in Escherichia coli JM109 cells
-
expressed in Escherichia coli strain CGSC7692 (pDTG601A)
-
expressed in Escherichia coli strain JM109
-
expression into Escherichia coli BW25113 DELTAgldA. A strongly regulated toluene dioxygenase plasmid system for the dearomatizing cis-1,2-dihydroxylation of benzene is developed. Escherichia coli BW25113 DELTAgldA strain harboring pBAD18-TDO system is a suitable platform for the production of the valuable compound cis-1,2-dihydrocatechol (DHC) at gram scale
expression of genes encoding toluene dioxygenase complex todC1C2BA in Escherichia coli
-
expression of mutant enzyme TDO 2-B38, in which the wild-type stop codon is replaced with a codon encoding Thr
-
expression of wild-type TodT, C-terminal part of TodT, and TodT mutants in Escherichia coli strain BL21(DE3). Genes todS and todT are transcribed from a single promoter called PtodX once the response regulator TodT is phosphorylated by the TodS sensor kinase in response to pathway substrates
-
genes encoding the three components of the toluene dioxygenase overproduced in Escherichia coli JM109: 1. the reductaseTOL - tolA, 2. the ferredoxinTOL and 3. the two subunits of the terminal dioxygenase - todC1C2
-
genes todC1C2BA, expression of the enzyme components todC1, C2, B, and A in Escherichia coli strain JM109 and in Rhodococcus opacus strain B-4. In Escherichia coli JM109, the yield of hydroxylated monoaromatics decreased with increasing substrate hydrophobicity, while in Rhodococcus opacus B-4, high performance in the hydroxylation of monoaromatics occurs, irrespective of substrate hydrophobicity.
-