1.14.12.22: carbazole 1,9a-dioxygenase
This is an abbreviated version!
For detailed information about carbazole 1,9a-dioxygenase, go to the full flat file.
Word Map on EC 1.14.12.22
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1.14.12.22
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ferredoxin
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rieske
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resinovorans
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carac
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carbazole-degradation
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dibenzo-p-dioxins
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dibenzofurans
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fluorene
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aromaticivorans
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nocardioides
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dianhydride
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dibenzothiophene
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polyimide
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janthinobacterium
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stipes
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degradation
- 1.14.12.22
- ferredoxin
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rieske
- resinovorans
- carac
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carbazole-degradation
- dibenzo-p-dioxins
- dibenzofurans
- fluorene
- aromaticivorans
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nocardioides
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dianhydride
- dibenzothiophene
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polyimide
- janthinobacterium
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stipes
- degradation
Reaction
Synonyms
CarAa, CarAc, carbazole dioxygenase, CARDO, CARDO-F, CARDO-R
ECTree
1.14.12.22 carbazole 1,9a-dioxygenaseAdvanced search results
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Engineering on EC 1.14.12.22 - carbazole 1,9a-dioxygenase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
F275A
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mutation in oxygenase component. Decrease in activity with carbazole, dibenzo-p-dioxin, anthracene, very low activity with fluorene, fluoranthene
F275I
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mutation in oxygenase component. Decrease in activity with carbazole, very low activtiy with dibenzo-p-dioxin, anthracene, fluorene, fluoranthene
F275L
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mutation in oxygenase component. Decrease in activity with carbazole, very low activtiy with dibenzo-p-dioxin, anthracene, fluorene, fluoranthene
F275V
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mutation in oxygenase component. Decrease in activity with carbazole, very low activtiy with dibenzo-p-dioxin, anthracene, fluorene, fluoranthene
F275W
F329A
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mutation in oxygenase component. Decrease in activity with carbazole, dibenzo-p-dioxin, anthracene, very low activity with fluorene, fluoranthene
F329I
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mutation in oxygenase component. Strong decrease in activity with all substrates tested
F329L
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mutation in oxygenase component. Strong decrease in activity with all substrates tested
F329V
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mutation in oxygenase component. Strong decrease in activity with all substrates tested
F329W
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mutation in oxygenase component. Strong decrease in activity with all substrates tested
I262A
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mutation in oxygenase component. Decrease in activity with carbazole, dibenzo-p-dioxin, anthracene, very low activity with fluorene, fluoranthene
I262L
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mutation in oxygenase component. Increase in activity with anthracene
I262V
I262W
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mutation in oxygenase component. Decrease in activity with carbazole, dibenzo-p-dioxin, anthracene, very low activity with fluorene, fluoranthene
Q282N
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mutation in oxygenase component. Decrease in activity with carbazole, very low activtiy with dibenzo-p-dioxin, anthracene, fluorene, fluoranthene
Q282S
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mutation in oxygenase component. Decrease in activity with carbazole, very low activtiy with dibenzo-p-dioxin, anthracene, fluorene, fluoranthene
Q282Y
degradation
additional information
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expression of the catalytic subunit CarAa is enhanced by induction at a lower cell concentration and temperature and over a longer time, while the expression of subunits CarAc and CarAd is inverse. Best co-expression condition tested is cell concentration at induction of 0.5 (absorbance at 600 nm), 37°C amd 4 h
F275W
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mutation in oxygenase component. Decrease in activity with carbazole, wild-type like activity with dibenzo-p-dioxin, anthracene, fluorene, fluoranthene
F275W
the mutant catalyzes the lateral dioxygenation of carbazole with higher yields than the wild type enzyme
I262V
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mutation in oxygenase component. Decrease in activity with carbazole, dibenzo-p-dioxin, anthracene, very low activity with fluorene, fluoranthene
I262V
the mutant catalyzes the lateral dioxygenation of carbazole with higher yields than the wild type enzyme
Q282Y
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mutation in oxygenase component. Decrease in activity with carbazole, very low activtiy with dibenzo-p-dioxin, anthracene, fluorene, fluoranthene
Q282Y
the mutant catalyzes the lateral dioxygenation of carbazole with higher yields than the wild type enzyme
degradation
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constitutive expression of terminal oxygenase subunit in Rhizobium tropici. In water-cultured siratro rhizospheres inoculated with the recombinant strain, 48% of the initial 25 microg dibenzofuran is removed within 3 days, and similar results are obtained in soil-cultured siratro rhizosperes using sterile vermiculite. In non-sterile field soils, the inoculated recombinant strain can grow on the siratro root in all soils tested, except for wet paddy field
degradation
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constitutive expression of terminal oxygenase subunit in Rhizobium tropici. In water-cultured siratro rhizospheres inoculated with the recombinant strain, 48% of the initial 25 microg dibenzofuran is removed within 3 days, and similar results are obtained in soil-cultured siratro rhizosperes using sterile vermiculite. In non-sterile field soils, the inoculated recombinant strain can grow on the siratro root in all soils tested, except for wet paddy field
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