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1.14.13.1: salicylate 1-monooxygenase

This is an abbreviated version!
For detailed information about salicylate 1-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.1

Reaction

salicylate
+
NADH
 +
H+
 +
O2
=
catechol
 +
NAD+
 +
H2O
 +
CO2

Synonyms

Efe-shyA, FAD/NADH-dependent SA 1-hydroxylase, FAD_binding_3 domain-containing protein, FGSG_03657, FgShy1, FgShyC, flavin-dependent monooxygenase salicylate hydroxylase, NahG, NahU, NRRL3_9723, oxygenase, salicylate 1-mono-, SA 1-decarboxylase/hydroxylase, SA 1-hydroxylase, SA hydroxylase, SA1H, SahA, salA, salcylic acid 1-hydroxylase, SALH, salicylate 1-hydroxylase, Salicylate 1-monooxygenase, salicylate hydroxylase, salicylate hydroxylase (decarboxylating), salicylate monooxygenase, salicylate, NADH: oxygen oxidoreductase, salicylate, NADH: oxygen oxidoreductase (1-hydroxylating, decarboxylating), salicylate-1-hydroxylase, salicylic acid hydroxylase, salicylic hydroxylase, SalOH, SHL, Shy1, ShyA, ShyC, SlSA 1H, SlSA1H, Solyc08g063130

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.1 salicylate 1-monooxygenase

Cloned

Cloned on EC 1.14.13.1 - salicylate 1-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
biotransformation experiments with resting cells of Escherichia coli JM109 harboring recombinant ahd genes reveal that AhdA2cA1c, AhdA1dA2d, and AhdA1eA2e can function as a salicylate 1-hydroxylase
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells
expressed in Escherichia coli strain BL21(DE3)
-
expressed in Escherichia coli XL1-Blue cells
-
expressed in Nicotiana tabacum
-
expression in Arabidopsis thalaina, with chloroplast targeting sequence
-
expression in Escherichia coli
-
gene nahG, recombinant expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3)
gene salA, DNA and amino acid sequence determination and analysis, phylogenetic analysis
gene salA, quantitative RT-PCR enzyme expression analysis
gene shy1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative RT-PCR enzyme expression analysis
gene shyA or NRRL3_9723, DNA and amino acid sequence determination and analysis, enzyme expression analysis, gene NRRL3_9723 shows high amino acid similarity to Shy1 from Fusarium graminearum (FgShy1) (65.1%) and to a putative salicylate hydroxylase (AN2114) from Aspergillus nidulans (77.3%), sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene shyC, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative RT-PCR enzyme expression analysis
gene Solyc08g063130, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, transient recombinant expression in Nicotiana benthamiana leaves
transgenic Populus tremula x Populus alba hybrid expressing the nahG transgene. Expression of nahG decreases quinic acid conjugates and increased catechol glucoside, while exerting little effect on levels of salicylic acid and catechol, the substrate and product, respectively, of the nahG enzyme
-