1.14.13.114: 6-hydroxynicotinate 3-monooxygenase

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For detailed information about 6-hydroxynicotinate 3-monooxygenase, go to the full flat file.

Reaction

Synonyms

6-hydroxynicotinic acid 3-monooxygenase, 6HNA monooxygenase, BB1770, BbNicC, NicC, PpNicC

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

1.14.13.114 6-hydroxynicotinate 3-monooxygenase

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Results	in table
509	AA Sequence
2	Application
6	Cloned(Commentary)
11	Cofactor
1	Crystallization (Commentary)
21	General Information
11	Inhibitors
18	kcat/KM [mM/s]
3	Ki Value [mM]
23	KM Value [mM]
2	Localization
2	Molecular Weight [Da]
11	Natural Substrates/ Products (Substrates)
53	Organism
4	Pathway
1	PDB ID
4	pH Optimum
1	pH Range
1	pH Stability
23	Protein Variants
4	Purification (Commentary)
8	Reaction
12	Reference
1	Specific Activity [micromol/min/mg]
25	Substrates and Products (Substrate)
7	Subunits
23	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
2	Temperature Stability [°C]
18	Turnover Number [1/s]

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Cloned on EC 1.14.13.114 - 6-hydroxynicotinate 3-monooxygenase

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expression in Escherichia coli

Pseudomonas fluorescens

697700

gene Bb1770, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strains MachI and BL21(DE3)

Bordetella bronchiseptica

A0A0H3LLR0

744353

gene Bb1770, recombinant overexpression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)

Bordetella bronchiseptica

A0A0H3LKL4

764159

gene nicC, DNA and amino acid sequence determination and analysis, sequence comparisons

Neobacillus niacini

744353

gene nicC, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strains MachI and BL21(DE3)

Pseudomonas putida

Q88FY2

744353

the nicC gene is cloned and expressed in Pseudomonas fluorescens (plasmid pIZNicC)

Pseudomonas putida

700940