1.14.13.122: chlorophyllide-a oxygenase

This is an abbreviated version!
For detailed information about chlorophyllide-a oxygenase, go to the full flat file.

Reaction

Synonyms

AtPTC52, CAO, CAO1, Cao2, CAO_1, chlorophyll a oxygenase, chlorophyll a oxygenase 1, chlorophyll a oxygenase/chlorophyll b synthase, chlorophyllide a oxygenase, chlorophyllide a oxygenase 1, EC 1.13.12.14, fch2, OsCAO1, Pchlide a oxygenase, PGL, protochlorophyllide a oxygenase, ZmCAO1

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.122 chlorophyllide-a oxygenase

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Results	in table
3	Activating Compound
29	AA Sequence
1	CAS Registry Number
26	Cloned(Commentary)
23	Cofactor
1	Expression
7	General Information
2	General Stability
6	Inhibitors
1	KM Value [mM]
27	Localization
9	Metals/Ions
6	Molecular Weight [Da]
64	Natural Substrates/ Products (Substrates)
38	Organism
5	Pathway
1	pI Value
48	Protein Variants
6	Purification (Commentary)
17	Reaction
38	Reference
1	Renatured (Commentary)
27	Source Tissue
3	Specific Activity [micromol/min/mg]
77	Substrates and Products (Substrate)
6	Subunits
58	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

Purification

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Purification on EC 1.14.13.122 - chlorophyllide-a oxygenase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Arabidopsis thaliana

Q8W496

764779

for immunoblotting analysis rosette leaves are homogenized with extraction buffer (50 mM Tris, pH 6.8, 2 mM EDTA, 10% glycerol, 2% SDS, 6% 2-mercaptoethanol), centrifugation, supernatant separated by SDS-PAGE, resolved proteins electroblotted onto Hybond-P membrane, identification of fusion enzyme signal with antibody-horseradish peroxidase system

Arabidopsis thaliana

-

700620

partially by purification of chloroplast envelope inner membrane and thylakoid membranes

Arabidopsis thaliana

-

663059

recombinant enzyme partially by purification of transgenic plant thylakoid membranes

Prochlorothrix hollandica

-

662456

refolded recombinant His6-tagged CAO from Escherichia coli by nickel affinity chromatography to about 85% purity

Arabidopsis thaliana

-

663257

rosette leaves are homogenized with extraction buffer (50 mM Tris, pH 6.8, 2 mM EDTA, 10% glycerol, 2% SDS, 6% 2-mercaptoethanol), centrifugation, supernatants separated by SDS-PAGE, proteins electroblotted onto a Hybond-P membrane, identification of fusion enzyme with antibody-horseradish peroxidase system

Arabidopsis thaliana

-

698958