1.14.13.163: 6-hydroxy-3-succinoylpyridine 3-monooxygenase
This is an abbreviated version!
For detailed information about 6-hydroxy-3-succinoylpyridine 3-monooxygenase, go to the full flat file.
Word Map on EC 1.14.13.163
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1.14.13.163
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pyrrolidine
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putida
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2,5-dihydroxypyridine
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tumefaciens
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agrobacterium
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arthrobacter
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fad-binding
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ochrobactrum
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wastes
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synthesis
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nicotine-degrading
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flavoprotein
- 1.14.13.163
- pyrrolidine
- putida
- 2,5-dihydroxypyridine
- tumefaciens
-
agrobacterium
- arthrobacter
-
fad-binding
-
ochrobactrum
- wastes
- synthesis
-
nicotine-degrading
- flavoprotein
Reaction
+ 2 NADH + 2 H+ + = + + 2 NAD+ +
Synonyms
6-hydroxy-3-succinoyl-pyridine 3-monooxygenase, 6-hydroxy-3-succinoylpyridine hydroxylase, AWN88_01205, Hsh, HSP 3-monooxygenase, HSP hydroxylase, HSP monooxygenase, hspA, hspB, HSPH, HSPHZZ, NADH-dependent HSP hydroxylase, NIC, nicotine hydroxylase, VppD, W7K_00670
ECTree
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Cloned
Cloned on EC 1.14.13.163 - 6-hydroxy-3-succinoylpyridine 3-monooxygenase
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DNA and amino acid sequence determination and analysis, sequence comparisons, RT-PCR expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene hspB, phylogenetic analysis and tree, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
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gene vppD cloned from a 97-kbp DNA fragment containing six nicotine degradation-related genes which is obtained by gap closing from the genome sequence of strain SJY1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
recombinant expression of His-tagged enzyme in Escherichia coli strain B21-Codon Plus(DE3)-RIL
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