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1.14.13.166: 4-nitrocatechol 4-monooxygenase

This is an abbreviated version!
For detailed information about 4-nitrocatechol 4-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.166

Reaction

4-nitrocatechol
+
NAD(P)H
 +
H+
 +
O2
=
2-hydroxy-1,4-benzoquinone
 +
nitrite
 +
NAD(P)+
 +
H2O

Synonyms

4-nitrophenol 4-monooxygenase/4-nitrocatechol 2-monooxygenase, NpcA, npcB, NpdA2, p-nitrophenol monooxygenase, p-nitrophenol monooxygenase-II, para-nitrophenol 4-monooxygenase, PdcA, pentachlorophenol-4-monooxygenase, PNP 4-monooxygenase, PNP monooxygenase, pnpA, two-component PNP monooxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.166 4-nitrocatechol 4-monooxygenase

Purification

Purification on EC 1.14.13.166 - 4-nitrocatechol 4-monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE-Sepharose column chromatography, Q-Sepharose column chromatography, and Sephacryl S-300 gel filtration
-
Ni-NTA agarose column chromatography
-
Ni-NTA agarose column chromatography and Sephadex G-100 gel filtration
Ni-NTA column chromatography