1.14.13.181: 13-deoxydaunorubicin hydroxylase
This is an abbreviated version!
For detailed information about 13-deoxydaunorubicin hydroxylase, go to the full flat file.
Reaction
Synonyms
CYP109A2, DoxA, DoxA monooxygenase
ECTree
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Inhibitors
Inhibitors on EC 1.14.13.181 - 13-deoxydaunorubicin hydroxylase
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dithiothreitol
strongly inhibitory, inhibition is reversible after desalting
doxorubicin
completely inhibits DoxA activity at both 1 and 5 mM, competitive with respect to daunorubicinol oxidation
quinidine
inhibits DoxA oxidation of daunorubicinol by 11% at 1 mM. At 5 mM, it inhibits at more than 95%
sulfaphenazole
inhibits DoxA oxidation of daunorubicinol by 20% at 1 mM. At 5 mM, it inhibits at more than 95%
troleandomycin
inhibits DoxA oxidation of daunorubicinol by 35% at 1 mM. At 5 mM, it inhibits at more than 95%
additional information
high ionic strength buffers containing 100 mM sodium phosphate, also strongly inhibits DoxA activity. This effect is partially reversible after exchange into low ionic strength buffers, such as 20 mM N-(2-hydroxyethyl)piperazine-N9-(2-ethanesulfonic acid) (HEPES) or 10 mM sodium phosphate (pH 7.5). No inhibition: 4-methylpyrazole (1 or 5 mM), rhodomycin D (1-5 mM)
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