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0.014 - 0.25
2,4-Dihydroxybenzoate
0.042 - 0.12
3,4-dihydroxybenzoate
0.15702 - 0.22802
3,4-dihydroxybenzoic acid
0.18
3-Chloro-4-hydroxybenzoate
-
-
0.0006 - 0.12
4-hydroxybenzoate
0.027
NADP+
-
reaction with 2,4-dihydroxybenzoate
additional information
additional information
-
0.014
2,4-Dihydroxybenzoate
-
-
0.25
2,4-Dihydroxybenzoate
-
-
0.042
3,4-dihydroxybenzoate
wild-type enzyme, pH 7.5, 30°C
0.048
3,4-dihydroxybenzoate
pH 8.0, 30°C, mutant DA015, with NADPH
0.052
3,4-dihydroxybenzoate
mutant L199V/Y385F, pH 7.5, 30°C
0.12
3,4-dihydroxybenzoate
pH 8.0, 30°C, mutant DA016, with NADPH
0.15702
3,4-dihydroxybenzoic acid
-
mutant Y385F/T294A, at pH 8.0 and 30°C
0.22802
3,4-dihydroxybenzoic acid
-
mutant Y385F, at pH 8.0 and 30°C
0.0006
4-hydroxybenzoate
-
pH 8, monomeric form
0.003
4-hydroxybenzoate
-
strain 557
0.0033
4-hydroxybenzoate
-
-
0.0072
4-hydroxybenzoate
-
-
0.0073
4-hydroxybenzoate
-
-
0.0079
4-hydroxybenzoate
-
strain IG
0.008
4-hydroxybenzoate
-
pH 8.0, 25°C, mutant enzyme D38Y/T42R
0.0081
4-hydroxybenzoate
-
strain 420
0.0095
4-hydroxybenzoate
-
reaction with NADH
0.0109
4-hydroxybenzoate
-
-
0.012
4-hydroxybenzoate
-
pH 8.0, 25°C, mutant enzyme D38A
0.014
4-hydroxybenzoate
-
pH 8.0, 25°C, mutant enzyme T42R
0.01955
4-hydroxybenzoate
-
mutant Y385F, at pH 8.0 and 30°C
0.02
4-hydroxybenzoate
-
wild-type enzyme
0.0213
4-hydroxybenzoate
-
-
0.022
4-hydroxybenzoate
-
-
0.022
4-hydroxybenzoate
mutant L199V/Y385F, pH 7.5, 30°C
0.024
4-hydroxybenzoate
pH 8.0, 30°C, mutant DA015, with NADPH
0.025
4-hydroxybenzoate
-
wild-type enzyme
0.025
4-hydroxybenzoate
-
pH 8, dimeric form
0.026
4-hydroxybenzoate
-
pH 8.0, 25°C, mutant enzyme D38Y
0.0264
4-hydroxybenzoate
pH 7.5, 30°C, recombinant enzyme PHBHCn2, via UV/VIS-NAD(P)H consumption determination, with NADPH
0.03
4-hydroxybenzoate
-
mutant enzyme H162R, H162Y, H162K, R269K, R269Y, R269N, R269S and R269T
0.0306
4-hydroxybenzoate
pH 7.5, 30°C, recombinant enzyme PHBHCn2, via product determination per HPLC, with NADPH
0.03467
4-hydroxybenzoate
-
wild type enzyme, at pH 8.0 and 30°C
0.036
4-hydroxybenzoate
-
pH 8.0, 25°C, wild-type enzyme
0.039
4-hydroxybenzoate
wild-type enzyme, pH 7.5, 30°C
0.04
4-hydroxybenzoate
-
mutant enzyme H162N, H162S, H162T and H162D
0.041
4-hydroxybenzoate
-
-
0.048
4-hydroxybenzoate
pH 8.0, 30°C, wild-type enzyme, with NADPH
0.04838
4-hydroxybenzoate
-
mutant Y385F/T294A, at pH 8.0 and 30°C
0.06
4-hydroxybenzoate
-
mutant enzyme R269D
0.075
4-hydroxybenzoate
-
mutant enzyme R42S
0.075
4-hydroxybenzoate
-
35°C
0.11
4-hydroxybenzoate
-
mutant enzyme R42K
0.12
4-hydroxybenzoate
pH 8.0, 30°C, mutant DA016, with NADPH
0.0122
NADH
-
strain 557
0.0226
NADPH
-
-
0.0227
NADPH
-
reaction with 4-hydroxybenzoate
0.025
NADPH
-
pH 8, dimeric form
0.03
NADPH
-
wild-type enzyme
0.0335
NADPH
-
strain 557
0.04
NADPH
-
pH 8.0, 25°C, mutant enzyme D38A
0.045
NADPH
-
pH 8.0, 25°C, mutant enzyme T42R
0.047
NADPH
-
pH 8.0, 25°C, mutant enzyme D38Y
0.05
NADPH
-
wild-type enzyme
0.05
NADPH
-
mutant enzyme H162R
0.052
NADPH
-
pH 8, monomeric form
0.0658
NADPH
-
strain 420
0.07
NADPH
-
mutant enzyme H162Y and R269K
0.084
NADPH
-
pH 8.0, 25°C, mutant enzyme D38Y/T42R
0.146
NADPH
pH 7.5, 30°C, recombinant enzyme PHBHCn2, via UV/VIS-NAD(P)H consumption determination
0.195
NADPH
-
pH 8.0, 25°C, wild-type enzyme
0.2
NADPH
-
mutant enzyme H162K
0.32
NADPH
-
mutant enzyme R269S
0.037
O2
-
-
0.05
O2
-
reaction with 2,4-dihydroxybenzoate
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
Km value for NADPH above 0.5 mM: mutant enzymes R269D, R269T, R269N, R269Y, H162D, H162T, H162S, H162N
-
additional information
additional information
apparent kinetic parameters are estimated through non-linear regression of the Michaelis-Menten equation
-
additional information
additional information
-
apparent kinetic parameters are estimated through non-linear regression of the Michaelis-Menten equation
-