1.14.13.212: 1,3,7-trimethyluric acid 5-monooxygenase
This is an abbreviated version!
For detailed information about 1,3,7-trimethyluric acid 5-monooxygenase, go to the full flat file.
Reaction
Synonyms
NADH-dependent trimethyluric acid monooxygenase, tmuM
ECTree
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General Information
General Information on EC 1.14.13.212 - 1,3,7-trimethyluric acid 5-monooxygenase
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evolution
genes involved in the caffeine C-8 oxidation pathway are located in a 25.2-kb genomic DNA fragment of CBB1, including cdhABC (coding for caffeine dehydrogenase) and tmuM (coding for TmuM), Physical map of genes for caffeine transformation in a 25.2-kb gene cluster in Pseudomonas sp. strain CBB1, overview
metabolism
additional information
the enzyme takes part in the caffeine C-8 oxidation pathway as the second enzyme, a NADH-dependent trimethyluric acid monooxygenase that catalyzes the conversion of trimethylurate to 1,3,7-trimethyl-5-hydroxyisourate. This product spontaneously decomposes to racemic 3,6,8-trimethylallantoin
metabolism
two distinct caffeine metabolic pathways in bacteria: N-demethylation and C-8 oxidation, the NADH-dependent trimethyluric acid monooxygenase is involved in the latter catalyzing the second step of the C-8 oxidation pathway, overview
homology models of trimethyluric acid monooxygenase against uric acid oxidase HpxO (which catalyzes uric acid to 5-hydroxyisourate) reveal a much bigger and hydrophobic cavity to accommodate the larger substrates
additional information
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homology models of trimethyluric acid monooxygenase against uric acid oxidase HpxO (which catalyzes uric acid to 5-hydroxyisourate) reveal a much bigger and hydrophobic cavity to accommodate the larger substrates