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1.14.13.217: protodeoxyviolaceinate monooxygenase

This is an abbreviated version!
For detailed information about protodeoxyviolaceinate monooxygenase, go to the full flat file.

Reaction

protodeoxyviolaceinate
+
NAD(P)H
+
O2
=
protoviolaceinate
+
NAD(P)+
+
H2O

Synonyms

protoviolaceinate synthase, VioD, VioD oxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.217 protodeoxyviolaceinate monooxygenase

Cloned

Cloned on EC 1.14.13.217 - protodeoxyviolaceinate monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
biosynthetic gene cluster vioABCD, DNA sequencing, transposon mutagenesis, and chemical analysis of the pathway intermediates produced heterologously in Escherichia coli
-
gene vioD, cloned under control of the PTAC promoter and inserted into the fuc locus of Escherichia coli strain BW25113, functional.expression of the vioABCE cluster from Chromobacterium violaceum strain ATCC 12472 under control of the inducible araC system and the vioD gene of Janthinobacterium lividum strain DSM 1522, which enables exclusive production of violacein in Escherichia coli K-12 MG1655 dVio-1
gene vioD, recombinant expression in Escherichia coli strain DH10B and Streptomyces albus strain J1074, coexpression with the other genes of the violacein biosynthesis pathway, reevaluation, overview
gene vioD, recombinant expression of His-tagged enzyme in Escherichia coli
gene vioD, reconstruction of the violacein biosynthesis pathway in Escherichia coli strain BL21 (DE3), recombinant expression of the C-terminally His6-tagged enzyme