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1.14.13.22: cyclohexanone monooxygenase

This is an abbreviated version!
For detailed information about cyclohexanone monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.22

Reaction

cyclohexanone
+
NADPH
 +
H+
 +
O2
=
hexano-6-lactone
 +
NADP+
 +
H2O

Synonyms

Bpro_556, CAMO, CHMO, ChnB, chnB protein, chnB1 protein, CHXON, CMO, cycloalkaone monooxygenase, cyclohexanone 1, 2-mono-oxygenase, cyclohexanone mono-oxygenase, cyclohexanone monooxygenase, cyclohexanone oxygenase, cyclohexanone:NADPH:oxygen oxidoreductase (lactone-forming), oxygenase, cyclohexanone mono-

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.22 cyclohexanone monooxygenase

Specific Activity

Specific Activity on EC 1.14.13.22 - cyclohexanone monooxygenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.4
-
substrate cyclohexanone, induced (1.0 mM isopropylthiogalactoside) cell culture extracts, modified Riesenberg medium, 30°C
0.5
-
substrate cyclohexanone, induced (1.0 mM isopropylthiogalactoside) cell culture extracts, BHI medium, 30°C
0.85
mutant W492A, cyclohexanone, 50 mM Tris-HCl buffer (pH 9.0), 0.1 mM NADPH, 25°C
1.4
-
H163Q mutant enzyme
14
purified recombinant His-tagged enzyme expressed in yeast
15
purified native enzyme
15.9
-
wild type enzyme
19.9
purified recombinant His-tagged enzyme expressed in E. coli
21
-
purified enzyme
24.75
purified His-tagged recombinant enzyme
3.57
with cyclohexanone as substrate, pH 7.5
3.68
-
with cyclohexanone as substrate, pH 7.5
3.75
with cyclohexanone as substrate, pH 7.5
5.4
-
H96Q mutant enzyme
6
wild type enzyme, cyclohexanone, 50 mM Tris-HCl buffer (pH 9.0), 0.1 mM NADPH, 25°C
6.8
substrate cyclohexanone, pH 8.0, 25°C
678
-
purified enzyme
8 - 10
-
partially purified enzyme, substrate 4-methylcyclohexanone
9.1
-
purified recombinant enzyme, substrate bicyclo[3.2.0]hept-2-en-6-one
9.2
-
purified native enzyme, substrate bicyclo[3.2.0]hept-2-en-6-one
9.9
-
purified enzyme
additional information