1.14.13.24: 3-hydroxybenzoate 6-monooxygenase

This is an abbreviated version!
For detailed information about 3-hydroxybenzoate 6-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.24

1.14.13.24

gentisate

flavoprotein

flavin

fad

rhodococcus

salicylate

hydroxylases

jostii

2,5-dihydroxybenzoate

maleylpyruvate

para-hydroxylation

alcaligenes

nadh-dependent

flavin-dependent

ncimb

near-uv

fumarylpyruvate

nadh-specific

p-hydroxybenzoate

regioselective

Reaction

Synonyms

3-HBA-6-hydroxylase, 3-hydroxybenzoate 6-hydroxylase, 3-hydroxybenzoate-6-hydroxylase, 3-hydroxybenzoic acid-6-hydroxylase, 3HB6H, m-hydroxybenzoate 6-hydroxylase, MNX2, Mnx2p, NagX, NarX, Ncgl2923, oxygenase, 3-hydroxybenzoate 6-mono-, XlnD

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

1.14.13.24 3-hydroxybenzoate 6-monooxygenase

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Results	in table
4	Activating Compound
1676	AA Sequence
1	Application
1	CAS Registry Number
10	Cloned(Commentary)
48	Cofactor
1	Crystallization (Commentary)
15	General Information
2	General Stability
26	Inhibitors
14	kcat/KM [mM/s]
44	KM Value [mM]
2	Localization
3	Metals/Ions
19	Molecular Weight [Da]
23	Natural Substrates/ Products (Substrates)
47	Organism
7	Pathway
5	PDB ID
13	pH Optimum
6	pH Range
1	pH Stability
2	Posttranslational Modification
19	Protein Variants
14	Purification (Commentary)
7	Reaction
4	Reaction Type
33	Reference
4	Source Tissue
11	Specific Activity [micromol/min/mg]
4	Storage Stability
80	Substrates and Products (Substrate)
19	Subunits
41	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
3	Temperature Range [°C]
1	Temperature Stability [°C]
19	Turnover Number [1/s]

Molecular Weight

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Molecular Weight on EC 1.14.13.24 - 3-hydroxybenzoate 6-monooxygenase

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108000

gel filtration

119000

recombinant His-tagged NarX, gel filtration

710986

125000

Corynebacterium glutamicum

Q8NLB6

recombinant Ncgl2923, gel filtration

712313

130000

Pseudomonas alcaligenes

Q9F131

His-tagged recombinant enzyme, native PAGE and gel filtration

674255

40000 - 45000

Burkholderia cepacia

gel filtration, SDS-PAGE

438955

41500

Klebsiella pneumoniae

gel filtration

438958

42000

Klebsiella pneumoniae

1 * 42000, SDS-PAGE

43000

44000

45000

3 * 45000, recombinant His-tagged NarX, SDS-PAGE

710986

47000

2 entries

49000

Corynebacterium glutamicum

Q8NLB6

3 * 49000, recombinant Ncgl2923, SDS-PAGE

712313

49900

Candida parapsilosis

x * 49900, calculated from amino acid sequence

67000

SDS-PAGE

70000

gel filtration

85000

Pseudomonas aeruginosa

sedimentation equilibrium measurement

438953