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1.14.13.25: methane monooxygenase (soluble)

This is an abbreviated version!
For detailed information about methane monooxygenase (soluble), go to the full flat file.

Word Map on EC 1.14.13.25

Reaction

methane
+
NAD(P)H
+
H+
+
O2
=
methanol
+
NAD(P)+
+
H2O

Synonyms

chcA, cytoplasmic methane monooxygenase, methane hydroxylase, methane mono-oxygenase, methane monooxygenase, methane monooxygenase hydroxylase, MmMmoC, MMO, MMO Bath, MMOB, MmoC, MMOH, MMOR, oxygenase, methane mono-, particulate methane monooxygenase, pMMO, sMMO, soluble methane monooxygenase, soluble methane monooxygenase hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.25 methane monooxygenase (soluble)

Renatured

Renatured on EC 1.14.13.25 - methane monooxygenase (soluble)

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RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme component MMOB from Escherichia coli is solubilized from the 12000 x g pellet with 8 M urea, followed by dilution to 1 mg/ml protein for enzyme refolding
-