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1.14.13.33: 4-hydroxybenzoate 3-monooxygenase [NAD(P)H]

This is an abbreviated version!
For detailed information about 4-hydroxybenzoate 3-monooxygenase [NAD(P)H], go to the full flat file.

Reaction

4-hydroxybenzoate
+
NAD(P)H
+
H+
+
O2
=
3,4-dihydroxybenzoate
+
NAD(P)+
+
H2O

Synonyms

4-HBA 3-hydroxylase, 4-HBA 3-monooxygenase, 4-hydroxybenzoate 3-hydroxylase, 4-hydroxybenzoate 3-monooxygenase (NAD(P)H), 4-hydroxybenzoate 3-monooxygenase (NAD(P)H2), 4-hydroxybenzoate-3-hydroxylase, 4HB 3-hydroxylase, NAD(P)H-dependent PHBHCn1, NADPH-dependent PHBH, oxygenase, 4-hydroxybenzoate 3-mono- (reduced nicotinamide adenine dinucleotide (phosphate)), p-hydroxybenzoate hydroxylase, para-hydroxybenzoate-3-hydroxylases, PHBH, PHBHCn1, PHBHRo, PHBHRo1CP, PHBHRr, PobA, PRAI, Reut_B5020, Xcc0356

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.33 4-hydroxybenzoate 3-monooxygenase [NAD(P)H]

Cloned

Cloned on EC 1.14.13.33 - 4-hydroxybenzoate 3-monooxygenase [NAD(P)H]

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene pobA, DNA and amino acid sequence determination and analysis and tree, sequence comparisons and phylogenetic analysis, recombinant expression of optimized enzyme, subcloning in Escherichia coli strain DH5alpha
gene praI, functional recombinant expression in different strains of Pseudomonas putida replacing the endogenous gene pobA that encodes a PHBH enzyme
gene Reut_B5020, DNA sequence comparisons and phylogenetic analysis and tree, recombinant expression of the codon-optimized gene in Acinetobacter sp. ADP1
praI gene expressed in Escherichia coli BL21(DE3) cells harboring pETI23
-