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1.14.13.38: anhydrotetracycline 6-monooxygenase

This is an abbreviated version!
For detailed information about anhydrotetracycline 6-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.38

Reaction

anhydrotetracycline
+
NADPH
+
H+
+
O2
=
12-dehydrotetracycline
+
NADP+
+
H2O

Synonyms

anhydrotetracycline hydroxylase, anhydrotetracycline monooxygenase, anhydrotetracycline oxygenase, ATC oxygenase, oxygenase, anhydrotetracycline, oxyS

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.38 anhydrotetracycline 6-monooxygenase

Cloned

Cloned on EC 1.14.13.38 - anhydrotetracycline 6-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene otcC, DNA and amino acid sequence determination and analysis, expression of the glycine mutant enzyme in Escherichia coli strain BL21(DE3)
gene oxyS, functional recombinant overexpression of C-terminally FLAG-tagged enzyme in Saccharomyces cerevisiae from plasmid pSP-G1 resulting in biosynthesis of dehydrotetracycline from anhydrotetracycline, functional coexpression with heterologous dehydrotetracycline reductase CtcM, and the F420 reductase FNO
-