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1.14.13.39: nitric-oxide synthase (NADPH)

This is an abbreviated version!
For detailed information about nitric-oxide synthase (NADPH), go to the full flat file.

Word Map on EC 1.14.13.39

Reaction

2 L-arginine + 2 NADPH + 2 H+ + 2 O2 = 2 Nomega-hydroxy-L-arginine + 2 NADP+ + 2 H2O

Synonyms

bacterial nitric oxide synthase, bacterial nitric-oxide synthase, bNOS, bsNOS, cb-NOS, cytokine inducible NOS, DNOS, e-NOS, EC-NOS, endothelial nitric oxide synthase, endothelial nitric-oxide synthase, endothelial NO synthase, endothelial NOS, endothelium-derived relaxation factor-forming enzyme, endothelium-derived relaxing factor synthase, eNOS, i-NOS, inducible nitric oxide synthase, inducible nitric-oxide synthase, inducible NO synthase, inducible NOS, iNOS, mitochondrial NO synthase, mitochondrial-specific nitric oxide synthase, mtNOS, n-NOS, NADPH diaphorase, NADPH-d, NADPH-diaphorase, NADPHd, neuronal nitric oxide synthase, neuronal nitric-oxide synthase, neuronal NO synthase, neuronal NOS, nicotinamide adenine dinucleotide phosphate-diaphorase, nitric oxid synthase, nitric oxide synthase, nitric oxide synthase-like protein, nitric oxide synthetase, nitric-oxide synthase, nNOS, nNOSalpha, NO synthase, NO synthase type I, NO synthase type II, NO synthase type III, NO-synthase, NOS, NOS I, NOS-2, NOS1, NOS2, NOS3, synthetase, nitric oxide

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.39 nitric-oxide synthase (NADPH)

Cloned

Cloned on EC 1.14.13.39 - nitric-oxide synthase (NADPH)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
bacterial NOS, phylogenetic tree, functional expression in Escherichia coli
cell-specific gene regulation mechanism of the endothelial isozyme in the vascular endothelium involving endothelial-specific promoter, binding sites for AP-1, high affinity Sp1-binding sites and GATA promoter sites, and several, e.g. octameric, transcriptional regulators, epigenetic regulatory mechanisms in vascular endothelial cell-specific gene expression, overview, the cell-specific chromatin structure regulates transcription, and transcription factors, e.g. Sp1, AP-1, GATA-2, and octamer KLF, regulate transcription via chromatin-remodeling activity, overlapping cis antisense gene, endothelial-specific genetic regulation model, overview
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co-expression of the enzyme and Vac14 as GST- or His-tagged proteins in Escherichia colis train BL21, expression of Vac14 as FLAG-tagged protein in HEK-293T cells
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DNA and amino acid sequence determination and analysis, functional expression of the C-terminally His6-tagged soluble enzyme in Escherichia coli strain BL21 (DE3), sequence alignment and comparative modeling
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endothelial enzyme expressed in Pichia pastoris using a highly inducible alcohol oxidase promoter PAOX1
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eNOS and iNOS expression analysis
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expressed in Escherichia coli BL21(DE3) cells
expressed in HEK-293 cells
expressed in SH-SY5Y cells
expression in Escherichia coli
expression in Escherichia coli strain BL21(DE3)
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expression in Escherichia coli, coexpression of calmodulin is necessary
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expression in HEK-293T cell
expression in HUVE cells, vascular smooth muscle cells, and in HeLa cells, DNA methylation of proximal promoter sequences of eNOS promoter-beta-Gal fusion contructs in trangenic mice is not essential for cell-specific expression, endothelial cells are highly enriched in acetylated H3 and acetylated H4 at the eNOS promoter altering the promoter function and expression
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expression of endothelial isoform III in Spodoptera frugiperda cells via baculovirus infection
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expression of endothelial isoform in human embryonic kidney cells
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expression of eNOS in Escherichia coli
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expression of GST-tagged splicing variants alpha and beta in HEK 293 cells
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expression of His-tagged and GST-tagged truncated nNOS, comprising residues 1-198, in Escherichia coli
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expression of His-tagged dNOS in Escherichia coli
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expression of His-tagged NOS oxygenase domain lacking the N-terminal 114 residues in Escherichia coli
expression of His-tagged wild-type enzyme and untagged truncation mutant enzyme in Escherichia coli strain BL21(DE3)
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expression of neuronal isoform wild type and mutants C415H, C415A in Spodoptera frugiperda cells via baculovirus infection
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expression of nNOS in Escherichia coli
expression of the C-terminally His-tagged iNOS oxygenase domain, iNOSoxy, in Escherichia coli strain BL21
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expression of the oxygenase domain of inducible nitric oxide synthase in Escherichia coli
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gene nos1, quantitative reverse-transcription PCR enzyme expression analysis
gene nos2, recombinant expression of wild-type and mutant enzymes in HEK293 cells or Escherichia coli
inducible liver isoform is expressed in Escherichia coli with and without His-tag, requires coexpression of calmodulin
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iNOS, DNA and amino acid sequence determination and analysis, phylogenetic analysis
native rat neuronal NOS reductase domain (nNOSred) and the R1400E variant are expressed from plasmid pCRNNR in Escherichia coli BL21(DE3)
neuronal enzyme expressed in Spodoptera frugiperda cells via baculovirus infection
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neuronal enzyme, stable expression in HEK-293 cells
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neuronal wild-type and natural mutant, specifically expressed in the central nervous system, expression in Escherichia coli and in insect cells of Spodoptera frugiperda via baculovirus infection
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overexpression of His6-tagged bNOS in Escherichia coli strain BL21(DE3)
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overexpression of wild-type endothelial enzyme and mutant endothelial enzyme S1179D in Escherichia coli
phylogenetic tree, expression of a His-tagged construct, dNOSr, comprising the dNOS reductase domain and its adjacent calmodulin binding site in Escherichia coli strain BL21
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rat brain enzyme expressed in human 293 kidney cells transfected with a vector encoding rat brain enzyme
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recombinant expression of bNOS
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stable, functional co-expression of enzyme and NMDA receptors in CHO1 cell plasma membranes, predominantly, and in the cytosol of CHO1(–) cells lacking postsynaptic density 95 proteins, PSD-95, expression
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the oxygenase domain of iNOS with a C-terminal His6-tag is expressed in Escherichia coli
the wild type and the DELTAG810 mutant of nNOS are cloned in Escherichia coli strain DH5alpha
transient expression of GFP-tagged isozyme NOS1 in COS-1 cell mitochondria, N-terminally truncated GFP-tagged isozyme NOS1 is localized in the cytosol
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untagged nNOS dimer protein is expressed using a recombinant baculovirus/Sf9 insect cell system
wild-type endothelial enzyme is expressed in insect cells via baculovirus infection
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