1.14.13.50: pentachlorophenol monooxygenase

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For detailed information about pentachlorophenol monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.50

1.14.13.50

pcp-degrading

flavobacterium

sphingobium

chlorophenolicum

tetrachlorohydroquinone

bioremediation

tetrachlorobenzoquinone

dehalogenation

environmental protection

arthrobacter

degradation

Reaction

Synonyms

CpsB, dechlorinase, pentachlorophenol, GM610_18120, oxygenase, pentachlorophenol 4-mono-, PCB hydroxylase, PCP 4-monooxygenase, PCP hydroxylase, PCP-4-monooxygenase, PCP-monooxygenase, PCP4MO, PcpB, PcpD, pentachlorophenol 4-mono-oxygenase, pentachlorophenol 4-monooxygenase, pentachlorophenol dechlorinase, pentachlorophenol dehalogenase, pentachlorophenol hydroxylase, pentachlorophenol monooxygenase, pentachlorophenol-4-monooxygenase, Phe4MO, tetrachlorobenzoquinone reductase

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

1.14.13.50 pentachlorophenol monooxygenase

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Results	in table
2	Activating Compound
821	AA Sequence
8	Application
2	CAS Registry Number
17	Cloned(Commentary)
30	Cofactor
1	Crystallization (Commentary)
14	General Information
1	Inhibitors
6	kcat/KM [mM/s]
16	KM Value [mM]
6	Localization
5	Metals/Ions
6	Molecular Weight [Da]
23	Natural Substrates/ Products (Substrates)
35	Organism
5	Pathway
2	PDB ID
7	pH Optimum
2	pH Range
1	pH Stability
1	pI Value
21	Protein Variants
11	Purification (Commentary)
6	Reaction
4	Reaction Type
30	Reference
1	Source Tissue
6	Specific Activity [micromol/min/mg]
3	Storage Stability
104	Substrates and Products (Substrate)
12	Subunits
55	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
2	Temperature Range [°C]
6	Temperature Stability [°C]
12	Turnover Number [1/s]

Purification

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Purification on EC 1.14.13.50 - pentachlorophenol monooxygenase

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2 entries

from culture extract to homogeneity, about 13fold

Pseudomonas fluorescens

658496

frozen pellet of cell culture is suspended in lysis buffer (50 mM Tris-HCl, pH 7.7, 025 mM phenylmethanesulfonyl fluoride, 1 microM pepstatin A, lysozyme), cells disrupted by sonication, centrifuged, supernatant purified by affinity chromatography, mixed with Ni-NTA agarose medium, washed, packed into a column, eluted, active fractions combined and stored in buffer (20 mM phosphate buffer, pH 7.0, 0.25 mM phenylmethanesulfonyl fluoride, 5% glycerol)

Sphingobium chlorophenolicum

partially

recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Bacillus tropicus

763935

recombinant enzyme from Escherichia coli to homogeneity

Sphingobium chlorophenolicum

659022

recombinant enzyme from Pseudomonas aeruginosa to over 95% homogeneity by nickel affinity chromatography

Sphingobium chlorophenolicum

659022

recombinant wild-type and mutant enzymes from Escherichia coli

Sphingobium chlorophenolicum

658210

recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)

Sphingobium chlorophenolicum

658439

recombinant wild-type and mutant from Escherichia coli as glutathione-S-transferase fusion proteins

Flavobacterium sp.

438820