1.14.13.59: L-lysine N6-monooxygenase (NADPH)

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For detailed information about L-lysine N6-monooxygenase (NADPH), go to the full flat file.

Reaction

Synonyms

EC 1.13.12.10, EC 13.12.10, flavin-dependent lysine monooxygenase, flavin-dependent N6-lysine monooxygenase, IucD, LH, lysine monooxygenase, Lysine N(6)-hydroxylase, Lysine N6-hydroxylase, lysine N6-monooxygenase, lysine: N6-hydroxylase, lysine:N(6)-hydroxylase, Lysine:N6-hydroxylase, MbsG, N-hydroxylating monooxygenase, NbtG, Oxygenase, lysine N6-mono-

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.59 L-lysine N6-monooxygenase (NADPH)

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Results	in table
4	Activating Compound
6102	AA Sequence
1	CAS Registry Number
5	Cloned(Commentary)
36	Cofactor
1	Crystallization (Commentary)
4	General Information
1	General Stability
10	Inhibitors
20	kcat/KM [mM/s]
1	Ki Value [mM]
38	KM Value [mM]
5	Localization
2	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
19	Organism
8	Pathway
4	PDB ID
7	pH Optimum
2	pH Range
25	Protein Variants
4	Purification (Commentary)
5	Reaction
2	Reaction Type
17	Reference
9	Specific Activity [micromol/min/mg]
1	Storage Stability
47	Substrates and Products (Substrate)
5	Subunits
29	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
28	Turnover Number [1/s]

Purification

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Purification on EC 1.14.13.59 - L-lysine N6-monooxygenase (NADPH)

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

recombinant enzyme form IucD439, in which the sequence encoding the IucD protein is fused in frame to the amino-terminal sequence of beta-galactosidase

Escherichia coli

-

207906

recombinant His-tagged wild-type and P14G mutant enzymes from strain M15-2 to homogeneity by gel filtration, ion exchange and nickel affinity chromatography

Escherichia coli

-

658232

recombinant His8-MBP-tagged wild-type and mutant K184A enzymes, as well as seleno-L-methionine-labeled enzyme from Escherichia coli TOP10 cells by nickel affinity chromatography, dialysis, tag cleavage by TEV protease, followed by a second step of nickel affinity chromatography and by MBP-tracking affinity chromatography, eluting the detagged enzyme, followed by dialysis

Nocardia farcinica

-

745322

recombinant wild-type and mutants from strain DH5alpha

Escherichia coli

-

658171