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0.38
1,1-Dimethylhydrazine
-
-
0.059
1,2,3,4-tetrahydroisoquinoline
-
-
12
1,2-dimethylhydrazine
-
-
0.08
1-Methyl-1-phenylhydrazine
-
-
2
1-methyl-2-benzylhydrazine
-
-
0.0018 - 0.137
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
0.117
10-(N,N-dimethylaminooctyl)2-(trifluoromethyl)phenothiazine
pH 8.4, 37°C
15 - 38
10-[(N,N-dimethylaminooctyl)-2-(trifluoromethyl)]phenothiazine
0.0711
4-(4-methoxyphenyl)-2-methylsulfanylmethyl-4-oxobutyric acid
-
pH 8.4, 37°C, recombinant FMO1
0.0032 - 0.0038
4-(4-methylphenyl)-2-methylsulfanylmethyl-4-oxobutyric acid
0.0229
5-[[3-(dimethylamino)propyl]amino]-8-hydroxy-6H-[1,2,3]triazolo[4,5,1-de]acridin-6-one
isozyme FMO1, in 0.1 M potassium phosphate buffer (pH 8.4) at 37°C
0.05903 - 0.4314
5-[[3-(dimethylamino)propyl]amino]-8-methoxy-6H-[1,2,3]triazolo[4,5,1-de]acridin-6-one
0.0186 - 0.0659
Benzydamine
3.3
beta-ethylphenylhydrazine
-
-
1
bicyclo[4.2.0]octan-7-one
-
at pH 8.0 and 20°C
0.408
bupivacaine
-
pH 8.0, 25°C
0.022 - 0.08
chlorpromazine
0.018 - 0.0444
clomiphene
2.1
Cyclobutanone
-
at pH 8.0 and 20°C
0.101 - 1.45
ethiofencarb
0.104 - 2.131
Ethionamide
0.0502 - 0.0761
ethylenethiourea
8.3
isopropylhydrazine
-
-
0.143
lidocaine
-
pH 8.0, 25°C
0.018 - 0.0527
mercaptoimidazole
0.007 - 0.5758
Methimazole
0.079
methiocarb
-
pH 9.0, isozyme FMO1
0.0048 - 10.2
methyl p-tolyl sulfide
0.0068 - 0.0808
N,N-dimethyl-8-[2-(trifluoromethyl)-10H-phenothiazin-10-yl]octan-1-amine
0.0445 - 0.261
N,N-dimethylamphetamine
0.018 - 0.232
N,N-Dimethylaniline
0.17
N-Aminohomopiperidine
-
-
0.61
N-aminomorpholine
-
-
0.03
N-Aminopiperidine
-
-
0.1
N-aminopyrrolidine
-
-
0.008
N-methyl-1,2,3,4-tetrahydroisoquinoline
-
-
0.01
NADH
-
with bicyclo[3.2.0]hept-2-en-6-one as cosubstrate, at pH 8.0 and 20°C
0.13
Nicotine
-
pH 8.5, 25°C
0.0871 - 0.0986
p-tolyl sulfide
0.21
propranolol
-
pH 8.0, 25°C
0.147
pyrazoloacridine
-
-
4.3 - 5.9
S-allyl-L-cysteine
0.0713 - 0.314
selegiline
0.31 - 0.35
seleno-L-methionine
0.0101 - 0.0163
sulindac sulfide
0.00134 - 0.121
tamoxifen
0.0058 - 0.147
thiacetazone
0.023
tozasertib
-
pH and temperature not specified in the publication
0.0015 - 0.58
trimethylamine
additional information
additional information
-
0.0018
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
-
-
0.006
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
-
-
0.038
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
-
-
0.137
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
-
isoform FMO3, pH and temperature not specified in the publication
15
10-[(N,N-dimethylaminooctyl)-2-(trifluoromethyl)]phenothiazine
-
pH 8.5, 37°C, detergent is Triton X-100, recombinant MBP-FMO3
25
10-[(N,N-dimethylaminooctyl)-2-(trifluoromethyl)]phenothiazine
-
pH 8.5, 37°C, detergent is Triton X-100, commercial recombinant FMO3
32
10-[(N,N-dimethylaminooctyl)-2-(trifluoromethyl)]phenothiazine
-
pH 8.5, 37°C, detergent is a minimal detergent, recombinant MBP-FMO3
35
10-[(N,N-dimethylaminooctyl)-2-(trifluoromethyl)]phenothiazine
-
pH 8.5, 37°C, detergent is a minimal detergent, commercial recombinant FMO3
38
10-[(N,N-dimethylaminooctyl)-2-(trifluoromethyl)]phenothiazine
-
pH 8.5, 37°C, detergent is CHAPS, recombinant MBP-FMO3
0.0032
4-(4-methylphenyl)-2-methylsulfanylmethyl-4-oxobutyric acid
-
pH 8.4, 37°C, recombinant FMO5
0.0038
4-(4-methylphenyl)-2-methylsulfanylmethyl-4-oxobutyric acid
-
pH 8.4, 37°C, recombinant FMO1
0.05903
5-[[3-(dimethylamino)propyl]amino]-8-methoxy-6H-[1,2,3]triazolo[4,5,1-de]acridin-6-one
isozyme FMO1, in 0.1 M potassium phosphate buffer (pH 8.4) at 37°C
0.4314
5-[[3-(dimethylamino)propyl]amino]-8-methoxy-6H-[1,2,3]triazolo[4,5,1-de]acridin-6-one
isozyme FMO5, in 0.1 M potassium phosphate buffer (pH 8.4) at 37°C
0.0186
Benzydamine
-
pH 7.4, 37°C
0.0345
Benzydamine
recombinant His-tagged full-length mutant E308G, pH 7.4, 37°C
0.0373
Benzydamine
recombinant His-tagged full-length mutant V257M, pH 7.4, 37°C
0.038
Benzydamine
recombinant His-tagged truncated mutant V257M, pH 7.4, 37°C
0.0397
Benzydamine
recombinant His-tagged truncated mutant E308G, pH 7.4, 37°C
0.0426
Benzydamine
-
pH 7.4, 37°C
0.052
Benzydamine
recombinant His-tagged full-length wild-type enzyme, pH 7.4, 37°C
0.053
Benzydamine
recombinant His-tagged truncated wild-type enzyme, pH 7.4, 37°C
0.056
Benzydamine
-
pH and temperature not specified in the publication
0.0593
Benzydamine
recombinant His-tagged full-length mutant E158K, pH 7.4, 37°C
0.0659
Benzydamine
recombinant His-tagged truncated mutant E158K, pH 7.4, 37°C
0.022
chlorpromazine
-
pH 8.4, 37°C, recombinant mutant H360P His6-tagged MBT-fusion-FMO1
0.026
chlorpromazine
-
pH 8.4, 37°C, recombinant mutant L360P His6-tagged MBT-fusion-FMO3
0.028
chlorpromazine
-
pH 8.4, 37°C, recombinant mutant L360H His6-tagged MBT-fusion-FMO3
0.058
chlorpromazine
-
pH 8.4, 37°C, recombinant wild-type His6-tagged MBT-fusion-FMO1
0.061
chlorpromazine
-
pH 8.4, 37°C, recombinant wild-type His6-tagged MBT-fusion-FMO3
0.076
chlorpromazine
-
pH 8.4, 37°C, recombinant mutant L360Q His6-tagged MBT-fusion-FMO3
0.08
chlorpromazine
-
pH 8.4, 37°C, recombinant mutant L360A His6-tagged MBT-fusion-FMO3
4.31
cimetidine
-
pH 8.3, S-oxygenation with (+)-enantiomer formation by isozyme FMO1
4.56
cimetidine
-
pH 8.3, S-oxygenation with (-)-enantiomer formation by isozyme FMO1
0.018
clomiphene
-
pH and temperature not specified in the publication
0.0183
clomiphene
recombinant wild-type enzyme, pH 7.4, 37°C
0.0205
clomiphene
recombinant mutant E158K, pH 7.4, 37°C
0.0332
clomiphene
recombinant mutant V257M, pH 7.4, 37°C
0.0444
clomiphene
recombinant mutant E308G, pH 7.4, 37°C
1.2
cysteamine
-
-
6
cysteamine
-
at pH 8.0 and 25°C
0.013
demeton-O
-
pH 9.0, isozyme FMO1
0.25
demeton-O
-
pH 9.0, isozyme FMO3
0.101
ethiofencarb
-
pH 9.0, isozyme FMO1
1.45
ethiofencarb
-
pH 9.0, isozyme FMO3
0.104
Ethionamide
37°C, isozyme FMO1
0.104
Ethionamide
isozyme FMO1, at pH 9.5 and 37°C
0.105
Ethionamide
37°C, isozyme FMO1
0.105
Ethionamide
isozyme FMO1, at pH 9.5 and 37°C
0.114
Ethionamide
37°C, isozyme FMO3
0.261
Ethionamide
37°C, isozyme FMO2
0.261
Ethionamide
isozyme FMO2, at pH 9.5 and 37°C
0.336
Ethionamide
37°C, isozyme FMO3
2.131
Ethionamide
37°C, isozyme FMO2
2.131
Ethionamide
isozyme FMO2, at pH 9.5 and 37°C
0.0502
ethylenethiourea
-
recombinant mutant K416N, pH 8.5
0.0557
ethylenethiourea
-
recombinant wild-type enzyme, pH 8.5
0.0761
ethylenethiourea
-
recombinant mutant E24D, pH 8.5
0.145
fenthion
-
wild-type enzyme, FMO3
0.15
fenthion
-
mutant enzyme D132H, FMO3
0.2
fenthion
-
mutant enzyme K158L, FMO3
0.22
fenthion
-
mutant enzyme K158L/D132H, FMO3
0.24
fenthion
-
mutant enzyme I303T, FMO1
0.3
fenthion
-
mutant enzyme R502X, FMO1
0.32
fenthion
-
mutant enzyme H97Q, FMO1
0.34
fenthion
-
wild-type enzyme, FMO1
0.351
fenthion
-
mutant enzyme I303V, FMO1
0.00457
GSK5182
recombinant mutant E158K, pH 7.4, 37°C
0.00982
GSK5182
recombinant wild-type enzyme, pH 7.4, 37°C
0.0285
GSK5182
recombinant mutant V257M, pH 7.4, 37°C
0.0587
GSK5182
recombinant mutant E308G, pH 7.4, 37°C
0.0047
imipramine
-
37°C
0.014
imipramine
-
mutant enzyme I303T, FMO1
0.014
imipramine
-
wild-type enzyme, FMO1
0.015
imipramine
-
mutant enzyme H97Q, FMO1
0.016
imipramine
-
mutant enzyme R502X, FMO1
0.02
imipramine
-
mutant enzyme I303V, FMO1
0.005
indole
wild-type, pH 8.0, temperature not specified in the publication
0.008
indole
mutant Y207S, pH 8.0, temperature not specified in the publication
0.09
indole
-
pH 8.5, 25°C
0.1
indole
-
pH 8.0, 25°C, recombinant Y207W
0.4
indole
-
pH 8.0, 25°C, recombinant wild-type enzyme
0.4
indole
-
pH 8.0, 25°C, recombinant C78A
0.4
indole
-
pH 8.0, 25°C, recombinant W319F
0.7
indole
-
pH 8.0, 25°C, recombinant C78V
0.8
indole
-
pH 8.0, 25°C, recombinant C78I
0.8
indole
-
pH 8.0, 25°C, recombinant C78I/Y207W/W319A
0.8
indole
-
pH 8.0, 25°C, recombinant C78L
0.8
indole
-
pH 8.0, 25°C, recombinant Y207W/W319A
0.9
indole
-
pH 8.0, 25°C, recombinant W319A
2.8
L-methionine
pH 7.6, 37°C, purified FMO3 from kidney
3.4
L-methionine
pH 7.6, 37°C, purified FMO3 from liver
6.5
L-methionine
-
recombinant FMO3, pH 7.4, 37°C
6.5
L-methionine
-
recombinant FMO3, pH 7.4, 37°C
10
L-methionine
-
above, recombinant FMO4, pH 7.4, 37°C
30
L-methionine
-
recombinant FMO2, pH 7.4, 37°C
48
L-methionine
-
recombinant FMO1, pH 7.4, 37°C
0.018
mercaptoimidazole
-
pH 8.4, 37°C, recombinant wild-type His6-tagged MBT-fusion-FMO3
0.02
mercaptoimidazole
-
pH 8.4, 37°C, recombinant mutant H360P His6-tagged MBT-fusion-FMO1
0.022
mercaptoimidazole
-
pH 8.4, 37°C, recombinant mutant L360Q His6-tagged MBT-fusion-FMO3
0.03
mercaptoimidazole
-
pH 8.4, 37°C, recombinant wild-type His6-tagged MBT-fusion-FMO1
0.034
mercaptoimidazole
-
pH 8.4, 37°C, recombinant mutant L360P His6-tagged MBT-fusion-FMO3
0.038
mercaptoimidazole
-
pH 8.4, 37°C, recombinant mutant L360H His6-tagged MBT-fusion-FMO3
0.0437
mercaptoimidazole
-
pH 8.4, 37°C, recombinant FMO3
0.046
mercaptoimidazole
-
pH 8.4, 37°C, recombinant mutant L360A His6-tagged MBT-fusion-FMO3
0.0527
mercaptoimidazole
-
pH 8.4, 37°C, recombinant FMO1
0.007
Methimazole
-
mutant enzyme I303V, FMO1
0.007
Methimazole
-
wild-type enzyme, FMO1
0.0081
Methimazole
9.5, 37°C, FMO1
0.01
Methimazole
pH 7.6, 37°C, purified FMO3 from liver
0.014
Methimazole
-
mutant enzyme H97Q, FMO1
0.016
Methimazole
-
mutant enzyme I303T, FMO1
0.0293
Methimazole
9.5, 37°C, FMO3
0.037
Methimazole
-
mutant W47F, pH 8.5, 25°C
0.04
Methimazole
pH 7.6, 37°C, purified FMO3 from kidney
0.066
Methimazole
-
pH 8.5, 25°C
0.0718
Methimazole
-
recombinant wild-type enzyme, pH 8.5
0.0773
Methimazole
-
recombinant mutant K416N, pH 8.5
0.0854
Methimazole
-
recombinant mutant E24D, pH 8.5
0.11
Methimazole
-
pH 8.0, 37°C
0.118
Methimazole
-
pH 8.0, 37°C, liver microsomes
0.1233
Methimazole
-
recombinant mutant N61K, pH 8.5
0.163
Methimazole
-
wild-type, pH 8.5, 25°C
0.5758
Methimazole
9.5, 37°C, FMO2.1
0.0048
methyl p-tolyl sulfide
pH 9.0, 37°C, mutant N413K
0.005
methyl p-tolyl sulfide
pH 9.0, 37°C, mutant S195L
0.0135
methyl p-tolyl sulfide
pH 9.0, 37°C, wild-type FMO2.1
0.2
methyl p-tolyl sulfide
-
mutant enzyme R502X, FMO1
0.245
methyl p-tolyl sulfide
-
mutant enzyme I303T, FMO1
0.251
methyl p-tolyl sulfide
-
mutant enzyme H97Q, FMO1
0.284
methyl p-tolyl sulfide
-
wild-type enzyme, FMO1
0.3
methyl p-tolyl sulfide
-
mutant enzyme I303V, FMO1
3.6
methyl p-tolyl sulfide
-
pH 8.5, FMO1
10.2
methyl p-tolyl sulfide
-
pH 8.5, FMO2
0.0068
N,N-dimethyl-8-[2-(trifluoromethyl)-10H-phenothiazin-10-yl]octan-1-amine
-
pH 8.4, 37°C, recombinant FMO1
0.0245
N,N-dimethyl-8-[2-(trifluoromethyl)-10H-phenothiazin-10-yl]octan-1-amine
-
pH 8.4, 37°C, recombinant FMO3
0.0808
N,N-dimethyl-8-[2-(trifluoromethyl)-10H-phenothiazin-10-yl]octan-1-amine
-
pH 8.4, 37°C, recombinant FMO5
0.0445
N,N-dimethylamphetamine
pH 7.4, 37°C, FMO1
0.261
N,N-dimethylamphetamine
pH 7.4, 37°C, FMO3
0.018
N,N-Dimethylaniline
-
-
0.02
N,N-Dimethylaniline
-
-
0.044
N,N-Dimethylaniline
-
-
0.232
N,N-Dimethylaniline
-
pH 8.5, 25°C
0.0031
NADPH
-
with bicyclo[3.2.0]hept-2-en-6-one as cosubstrate, at pH 8.0 and 20°C
0.0041
NADPH
-
wild-type, pH 8.5, 25°C
0.0043
NADPH
-
mutant W47F, pH 8.5, 25°C
0.0068
NADPH
pH 9.0, 37°C, mutant N413K
0.0115
NADPH
pH 9.0, 37°C, wild-type FMO2.1
0.013
NADPH
-
pH 8.5, 25°C
0.028
NADPH
-
with dimethylaniline
0.05
NADPH
-
with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine
0.132
NADPH
pH 9.0, 37°C, mutant S195L
0.019
O2
-
wild-type, pH 8.5, 25°C
0.042
O2
-
mutant W47F, pH 8.5, 25°C
0.043
O2
-
with dimethylaniline
0.049
O2
-
with dimethylaniline
0.0871
p-tolyl sulfide
-
37°C
0.0986
p-tolyl sulfide
-
37°C
4.3
S-allyl-L-cysteine
pH 7.6, 37°C, purified FMO3 from kidney
5.9
S-allyl-L-cysteine
pH 7.6, 37°C, purified FMO3 from liver
0.0713
selegiline
-
pH 8.0
0.1775
selegiline
-
pH 7.4
0.314
selegiline
-
pH and temperature not specified in the publication
0.31
seleno-L-methionine
pH 7.6, 37°C, purified FMO3 from liver
0.35
seleno-L-methionine
pH 7.6, 37°C, purified FMO3 from kidney
0.0665
sulindac
-
recombinant mutant K416N, pH 8.5
0.0693
sulindac
-
pH 9.0, 37°C
0.1208
sulindac
-
recombinant mutant E24D, pH 8.5
0.1501
sulindac
-
recombinant wild-type enzyme, pH 8.5
0.2071
sulindac
-
recombinant mutant N61K, pH 8.5
0.0101
sulindac sulfide
recombinant His-tagged full-length mutant E308G, pH 7.4, 37°C
0.0103
sulindac sulfide
recombinant His-tagged truncated mutant E308G, pH 7.4, 37°C
0.0103
sulindac sulfide
recombinant His-tagged truncated mutant V257M, pH 7.4, 37°C
0.0127
sulindac sulfide
recombinant His-tagged truncated wild-type enzyme, pH 7.4, 37°C
0.0143
sulindac sulfide
recombinant His-tagged full-length mutant V257M, pH 7.4, 37°C
0.0151
sulindac sulfide
recombinant His-tagged full-length mutant E158K, pH 7.4, 37°C
0.0156
sulindac sulfide
recombinant His-tagged full-length wild-type enzyme, pH 7.4, 37°C
0.0163
sulindac sulfide
recombinant His-tagged truncated mutant E158K, pH 7.4, 37°C
0.00134
tamoxifen
recombinant His-tagged truncated mutant E158K, pH 7.4, 37°C
0.00156
tamoxifen
recombinant mutant E158K, pH 7.4, 37°C
0.0016
tamoxifen
recombinant His-tagged full-length mutant E158K, pH 7.4, 37°C
0.0025
tamoxifen
recombinant His-tagged full-length mutant E308G, pH 7.4, 37°C
0.0025
tamoxifen
recombinant mutant E308G, pH 7.4, 37°C
0.0029
tamoxifen
recombinant His-tagged truncated mutant E308G, pH 7.4, 37°C
0.005
tamoxifen
-
pH and temperature not specified in the publication
0.0056
tamoxifen
recombinant His-tagged truncated wild-type enzyme, pH 7.4, 37°C
0.0064
tamoxifen
recombinant wild-type enzyme, pH 7.4, 37°C
0.0064
tamoxifen
recombinant His-tagged full-length wild-type enzyme, pH 7.4, 37°C
0.0071
tamoxifen
recombinant His-tagged truncated mutant V257M, pH 7.4, 37°C
0.0081
tamoxifen
recombinant His-tagged full-length mutant V257M, pH 7.4, 37°C
0.0081
tamoxifen
recombinant mutant V257M, pH 7.4, 37°C
0.043
tamoxifen
-
pH 8.5, 37°C, recombinant isozyme FMO1 and recombinant mutant isozyme FMO3 E158K
0.121
tamoxifen
-
pH 8.5, 37°C, recombinant wild-type isozyme FMO3
0.0058
thiacetazone
9.5, 37°C, FMO2.1
0.0063
thiacetazone
9.5, 37°C, FMO1
0.007
thiacetazone
9.5, 37°C, FMO3
0.131
thiacetazone
-
recombinant EtaA, pH 9.0, 37°C, thiacetazone sulfinic acid formation
0.147
thiacetazone
-
recombinant EtaA, pH 9.0, 37°C, thiacetazone carbodiimide formation
0.027
Thiourea
-
-
0.0385
Thiourea
-
at pH 8.0 and 30°C
0.38
Thiourea
-
at pH 8.0 and 25°C
0.0015
trimethylamine
-
mutant W47F, pH 8.5, 25°C
0.0026
trimethylamine
-
wild-type, pH 8.5, 25°C
0.00875
trimethylamine
-
pH 8.4, 37°C, recombinant FMO3
0.0202
trimethylamine
-
at pH 8.0 and 30°C
0.0209
trimethylamine
-
recombinant wild-type enzyme, pH 8.5
0.0228
trimethylamine
-
recombinant mutant E24D, pH 8.5
0.0373
trimethylamine
-
recombinant mutant K416N, pH 8.5
0.073
trimethylamine
-
pH 8.5, 25°C
0.58
trimethylamine
-
at pH 8.0 and 25°C
3
voriconazole
-
pH 7.4, 37°C, recombinant FMO1
3.4
voriconazole
-
pH 7.4, 37°C, recombinant FMO3
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
2 Km values for oxygenation of thiocarbamides: 1. Km1 for oxygenation to sulfenic acid, 2. Km2 for oxygenation of sulfenic acid to sulfinic acid
-
additional information
additional information
-
2 Km values for oxygenation of thiocarbamides: 1. Km1 for oxygenation to sulfenic acid, 2. Km2 for oxygenation of sulfenic acid to sulfinic acid
-
additional information
additional information
-
steady-state kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics, overview
-
additional information
additional information
-
Michaelis-Menten kinetics, overview
-
additional information
additional information
-
Michaelis-Menten kinetics, overview
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additional information
additional information
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comparison of kinetics of recombinant isozymes FMO1 and FMO3
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additional information
additional information
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kinetics of wild-type and mutant structural variants
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additional information
additional information
kinetics for kidney and liver enzymes, overview
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additional information
additional information
MichaelisMenten kinetics
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additional information
additional information
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MichaelisMenten kinetics
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additional information
additional information
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the recombinant enzyme exhibits Michaelis-Menten kinetics
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additional information
additional information
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the recombinant enzyme exhibuts Michaelis-Menten kinetics
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additional information
additional information
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kinetic analysis of commercial recombinant enzymes and recombinant MBP-FMOs expressed in Escherichia coli, overview
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additional information
additional information
Michaelis-Menten steady-state kinetics
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additional information
additional information
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Michaelis-Menten steady-state kinetics
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additional information
additional information
Michaelis-Menten kinetics, potentiometric titrations of the flavin cofactor of FMO3 and the calculated reduction potentials for full-length wild-type and polymorphic variants of FMO3 and that of the truncated form of the wild-type
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additional information
additional information
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Michaelis-Menten kinetics, potentiometric titrations of the flavin cofactor of FMO3 and the calculated reduction potentials for full-length wild-type and polymorphic variants of FMO3 and that of the truncated form of the wild-type
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additional information
additional information
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steady-state kinetic analyses, overview
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