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1.14.13.84: 4-hydroxyacetophenone monooxygenase

This is an abbreviated version!
For detailed information about 4-hydroxyacetophenone monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.84

Reaction

(4-hydroxyphenyl)ethan-1-one
+
NADPH
+
H+
+
O2
=
4-hydroxyphenyl acetate
+
NADP+
+
H2O

Synonyms

4-hydroxyacetophenone monooxygenase, arylketone monooxygenase, Baeyer-Villiger monooxygenase, hAPA, HAPMO, More, oxygenase, 4-hydroxyacetophenone mono-

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.84 4-hydroxyacetophenone monooxygenase

KCat KM Value

KCat KM Value on EC 1.14.13.84 - 4-hydroxyacetophenone monooxygenase

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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.7
2,4-Pentanedione
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
110
2-acetylpyrrole
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
4.4
2-decanone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
176
2-hydroxyacetophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
0.4
2-phenylpropionaldehyde
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
32.7
3'-hydroxyacetophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
3.3
3-aminoacetophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
4.3
3-methoxyacetophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
4.9
3-nitroacetophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
1450
4-aminoacetophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
11
4-chloroacetophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
6.2
4-fluoroacetophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
257
4-hydroxyacetophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
5.9
4-methoxyacetophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
18.5
4-methylacetophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
8.4
4-Nitroacetophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
41.6
acetophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
8.1
benzaldehyde
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
0.2
butyrophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
1.1
methyl-4-tolyl sulfide
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
28.5
propiophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
138
trifluoroacetophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C