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1.14.13.9: kynurenine 3-monooxygenase

This is an abbreviated version!
For detailed information about kynurenine 3-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.9

Reaction

L-kynurenine
+
NADPH
 +
H+
 +
O2
=
3-hydroxy-L-kynurenine
 +
NADP+
 +
H2O

Synonyms

BcKMO, Bna4, cinnabar, EC 1.14.1.2, EC 1.99.1.5, FAD dependent kynurenine 3-monooxygenase, flavin adenine dinucleotide dependent kynurenine 3-monooxygenase, hKMO, Hs-KMO, K3H, KMO, KYN-OHase, kynurenine 3-hydroxylase, kynurenine 3-monooxygenase, kynurenine hydroxylase, kynurenine monooxygenase, kynurenine-3-monooxygenase, L-kynurenine 3-monooxygenase, L-kynurenine,NADPH2:oxygen oxidoreductase (3-hydroxylating), L-kynurenine-3-hydroxylase, More, NADPH-dependent flavin monooxygenase, oxygenase, kynurenine 3-mono-, pfKMO, Rat-KMO, scKMO

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.9 kynurenine 3-monooxygenase

Purification

Purification on EC 1.14.13.9 - kynurenine 3-monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography
-
fusion protein with gluthatione-S-transferase
-
partial
recombinant enzyme 8.8fold from Escherichia coli strain by ammonium sulfate fractionation and ion exchange chromatography to homogeneity
-
recombinant enzyme from Sf9 insect cells by solubilization with Triton X-100 and affinity chromatography
recombinant FLAG/GST-tagged full-length KMO (1-478) from Spodoptera frugiperda (Sf9) cells by glutathione affinity chromatography, ultrafiltration, and desalting gel filtration, followed by tag cleavage through thrombin, gel filtration, anti-FLAG affinity imuno-chromatography, and again ultrafiltration and gel filtration
recombinant His-tagged enzyme from soluble fraction of Sf9 insect cells by nickel affinity chromatography