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1.14.13.92: phenylacetone monooxygenase

This is an abbreviated version!
For detailed information about phenylacetone monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.92

Reaction

phenylacetone
+
NADPH
+
H+
+
O2
=
Benzyl acetate
+
NADP+
+
H2O

Synonyms

4-hydroxyacetophenone monooxygenase, Baeyer-Villiger monooxygenase, BVMO, EtaA, HAPMO, M-PAMO, More, PAMO, phenylacetone monooxygenase, Tf PAMO

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.92 phenylacetone monooxygenase

Purification

Purification on EC 1.14.13.92 - phenylacetone monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Q-Sepharose, hydroxyapatite, Superdex 200
-
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by one-step nickel affinity chromatography to near-homogeneity
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli
recombinant His-tagged wild-type and mutant PAMOs from Escherichia coli strain TOP10 by nickel affinity chromatography
-
recombinant PAMO