1.14.13.92: phenylacetone monooxygenase

This is an abbreviated version!
For detailed information about phenylacetone monooxygenase, go to the full flat file.

Reaction

Synonyms

4-hydroxyacetophenone monooxygenase, Baeyer-Villiger monooxygenase, BVMO, EtaA, HAPMO, M-PAMO, More, PAMO, phenylacetone monooxygenase, Tf PAMO

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.92 phenylacetone monooxygenase

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Results	in table
3	Activating Compound
413	AA Sequence
9	Application
1	CAS Registry Number
13	Cloned(Commentary)
38	Cofactor
2	Crystallization (Commentary)
9	General Information
2	General Stability
16	Inhibitors
42	kcat/KM [mM/s]
69	KM Value [mM]
1	Localization
1	Molecular Weight [Da]
29	Natural Substrates/ Products (Substrates)
6	Organic Solvent Stability
69	Organism
13	PDB ID
16	pH Optimum
3	pH Range
1	Posttranslational Modification
112	Protein Variants
10	Purification (Commentary)
7	Reaction
2	Reaction Type
29	Reference
3	Specific Activity [micromol/min/mg]
131	Substrates and Products (Substrate)
4	Subunits
49	Synonyms
1	Systematic Name
21	Temperature Optimum [°C]
1	Temperature Range [°C]
6	Temperature Stability [°C]
62	Turnover Number [1/s]

Temperature Stability

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Temperature Stability on EC 1.14.13.92 - phenylacetone monooxygenase

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

50

Thermobifida fusca

Q47PU3

PAMO exhibits high thermostability and activity at 50°C

764409

52

Thermobifida fusca

-

50% loss of activity after 24 h and 48 h in the absence and presence of FAD, respectively

657625

57.5

Thermobifida fusca

Q47PU3

Tm value for enzyme PAMO mutant A442P/L443V

744548

58.5

Thermobifida fusca

Q47PU3

Tm value for enzyme PAMO mutant S441G/A442P/L443T/S444Q

744548

60

Thermobifida fusca

-

the wild type enzyme has a melting temperature of 60°C

726793

60.5

Thermobifida fusca

Q47PU3

Tm value for wild-type enzyme PAMO

744548