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1.14.14.12: 3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase

This is an abbreviated version!
For detailed information about 3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase, go to the full flat file.

Reaction

3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione
+
FMNH2
+
O2
=
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione
+
FMN
+
H2O

Synonyms

3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione 4-hydroxylase, HsaAB

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.12 3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase

Crystallization

Crystallization on EC 1.14.14.12 - 3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor-diffusion method at 18°C, the crystal structure of HsaA to 2.5 A resolution reveales that the enzyme has the same fold, flavin-binding site, and catalytic residues as p-hydroxyphenyl acetate hydroxylase. HsaA prepared aerobically from the Escherichia coli host exists as a mixture of dimers and octamers in solution while HsaA prepared anaerobically from the rhodococcal host exists as a tetramer