1.14.14.23: cholesterol 7alpha-monooxygenase
This is an abbreviated version!
For detailed information about cholesterol 7alpha-monooxygenase, go to the full flat file.
Word Map on EC 1.14.14.23
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1.14.14.23
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lipoprotein
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hepatocytes
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triglyceride
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biliary
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hmg-coa
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farnesoid
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fecal
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low-density
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hypercholesterolemia
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cholic
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high-fat
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chenodeoxycholic
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3-hydroxy-3-methylglutaryl
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ba
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cyp8b1
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cholestyramine
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enterohepatic
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cholestasis
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hdl
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gallstone
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hypocholesterolemic
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27-hydroxylase
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oxysterols
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cholesteryl
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hmgcr
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cyp27a1
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taurocholate
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cholesterol-lowering
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hypolipidemic
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ldl-cholesterol
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acyl-coa:cholesterol
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27-hydroxycholesterol
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alpha-hydroxylation
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lithogenic
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ursodeoxycholic
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lxralpha
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hnf4alpha
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cyp3a11
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sult2a1
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4alpha
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cholesterogenesis
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gut-liver
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npc1l1
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cholesterol-free
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srebp2
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ldl-receptor
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c1-like
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medicine
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analysis
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obeticholic
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cholestanol
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guggulsterone
- 1.14.14.23
- lipoprotein
- hepatocytes
- triglyceride
- biliary
- hmg-coa
-
farnesoid
-
fecal
-
low-density
- hypercholesterolemia
-
cholic
-
high-fat
-
chenodeoxycholic
-
3-hydroxy-3-methylglutaryl
- ba
- cyp8b1
- cholestyramine
-
enterohepatic
- cholestasis
- hdl
- gallstone
-
hypocholesterolemic
-
27-hydroxylase
- oxysterols
-
cholesteryl
- hmgcr
- cyp27a1
- taurocholate
-
cholesterol-lowering
-
hypolipidemic
-
ldl-cholesterol
-
acyl-coa:cholesterol
- 27-hydroxycholesterol
-
alpha-hydroxylation
-
lithogenic
-
ursodeoxycholic
-
lxralpha
-
hnf4alpha
- cyp3a11
- sult2a1
- 4alpha
-
cholesterogenesis
-
gut-liver
-
npc1l1
-
cholesterol-free
-
srebp2
-
ldl-receptor
-
c1-like
- medicine
- analysis
-
obeticholic
- cholestanol
-
guggulsterone
Reaction
Synonyms
7alpha-hydroxylase, bile acid-synthetic enzyme, C7alphaOH, cholesterol 7 alpha-hydroxylase, cholesterol 7-alpha hydroxylase, Cholesterol 7-alpha-hydroxylase, Cholesterol 7-alpha-monooxygenase, cholesterol 7a-hydroxylase, cholesterol 7alpha hydroxylase, cholesterol 7alpha-hydroxylase, cholesterol-NADPH oxidoreductase, 7alpha-hydroxylating, CYP125A4, CYP7A, CYP7A1, Cyp7alpha1, CYPVII, cytochrome P450 125A4, EC 1.14.13.17, hepatic cholesterol 7alpha-hydroxylase, More, oxygenase, cholesterol 7alpha-mono-
ECTree
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Crystallization
Crystallization on EC 1.14.14.23 - cholesterol 7alpha-monooxygenase
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wild-type CYP7A1 in the ligand-free state, structure-based mutation T104L in the B' helix, corresponding to the nonpolar residue of CYP7B1, is used to obtain crystals of complexes with cholest-4-en-3 -one and with cholesterol oxidation product 7-ketocholesterol, hanging drop vapor diffusion method, mixing 0f 0.001 ml of protein solution with 0.001 ml of reservoir solution containing 0.1 M sodium chloride, 0.1 M trisodium citrate, pH 5.5, and 20% PEG 400, 18°C, to obtain a complex with cholest-4-en-3-one, a T104L mutant is used, and the crystals are grown in conditions containing 0.1 M MES, pH 6.0, and 18% PEG 550 monomethylether and soaked with 20% glycerol. For the T104L mutant-7KCh complex, crystals are grown in 0.1 M sodium chloride, 0.1 M tri-sodium citrate, pH 5.6, and 20% PEG 400, room temperature, X-ray diffraction structure determination and analysis at 1.90-2.75 A resolution