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1.14.15.4: steroid 11beta-monooxygenase

This is an abbreviated version!
For detailed information about steroid 11beta-monooxygenase, go to the full flat file.

Word Map on EC 1.14.15.4

Reaction

A steroid
+ 2 reduced adrenodoxin +
O2
 + 2 H+ =
an 11beta-hydroxysteroid
 + 2 oxidized adrenodoxin +
H2O

Synonyms

11-hydroxylase, 11 beta-hydroxylase, 11-beta hydroxylase, 11-beta-hydroxylase, 11-hydroxylase, 11beta hydroxylase, 11beta-hydroxylase, ALDOS, Aldosterone synthase, Aldosterone-synthesizing enzyme, C450XIB2, CYP11B1, CYP11B2, cyp11beta, CYPXIB, CYPXIB1, CYPXIB2, CYPXIB3, cytochrome 11beta-hydroxylase, cytochrome P-45011-beta, cytochrome P450 11B1, cytochrome P450 11B2, cytochrome P450 11beta-hydroxylase, cytochrome P45011beta, cytochrome P45011beta-hydroxylase, EC 1.14.1.6, EC 1.99.1.7, P-450(11 beta,aldo), P-450Aldo, P-450c11, P-450C18, P-450XIB1, P450 11beta-hydroxylase, P450(11 beta)-DS, P45011beta, P450C11, Steroid 11-beta-hydroxylase, steroid 11-hydroxylase, steroid 11beta-hydroxylase, steroid 11beta-monooxygenase, steroid 11beta/18-hydroxylase, Steroid 18-hydroxylase, steroid-11beta-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.15.4 steroid 11beta-monooxygenase

Purification

Purification on EC 1.14.15.4 - steroid 11beta-monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate fractionation, ion-exchange chromatography
-
ammonium sulfate precipitation, octyl-Sepharose adsorption with cholate and phosphotidylcholine extraction
-
cholate extraction, hydroxyapatite HPLC
-
copurification of 11beta-/18-hydroxylase with 11beta-/19-hydroxylase, cholate extraction
-
copurification of 11beta-/18-hydroxylase, cholate extraction, ammonium sulfate precipitation, Tween extraction, octymine-Sepharose
-
copurification of 11beta-hydroxylase with 18-hydroxylase, ammonium sulfate, DEAE-chromatography
-
copurification of P-450 11beta-2 and 11beta-3, ammonium sulfate precipitation, octyl-Sepharose, separation of 2 isoforms by hydroxyapatite column connected to an FPLC system
-
Na-cholate extraction, ammonium sulfate precipitation, aniline-Sepharose chromatography, hydroxylapatite HPLC
-
native enzyme by anion exchange and hydrophobic interaction chromatography, and two additonal steps of anion exchange chromatography
-
octyl-Sepharose chromatography, cholate extraction
-
recombinant C-terminally His6-tagged CYP11B2 from Escherichia coli by nickel affinity chromatography and anion and cation exchange chromatography
recombinant enzyme from Escherichia coli to homogeneity
-
recombinant mature form of C-terminally His6-tagged CYP11B1 from Escherichia coli strain JM109 by nickel affinity and cation exchange chromatography to homogeneity
recombinant mature form of C-terminally His6-tagged CYP11B2 from Escherichia coli strain JM109 by nickel affinity and cation exchange chromatography to homogeneity
RP HPLC for detection of photolabeled peptide after digest with TPCK-treated trypsin of purified cytochrome P-45011beta, which is photolabeled with radioactive methyltrienolone
-
sonication, acetone and butanol extraction, Triton extraction
-
Triton X-100, sodium cholate, column chromatographies
-
ultracentrifugation, ammonium sulfate precipitation, omega-aminooctyl-Sepharose, adrenodoxin-Sepharose, no glycerol used
-
without ionic detergents, ammonium sulfate fractionation, hexyl agarose
-