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1.14.15.6: cholesterol monooxygenase (side-chain-cleaving)

This is an abbreviated version!
For detailed information about cholesterol monooxygenase (side-chain-cleaving), go to the full flat file.

Word Map on EC 1.14.15.6

Reaction

(20R,22R)-20,22-dihydroxy-cholesterol
+ 2 reduced adrenodoxin +
O2
+ 2 H+ =
pregnenolone
+
4-Methylpentanal
+ 2 oxidized adrenodoxin + 2 H2O

Synonyms

C27-side chain cleavage enzyme, cholesterol 20-22-desmolase, cholesterol C20-22 desmolase, cholesterol C20-C22 lyase, cholesterol desmolase, cholesterol hydroxylase, cholesterol side chain cleavage cytochrome P450, cholesterol side chain cleavage enzyme, cholesterol side-chain cleavage cytochrome P450, cholesterol side-chain cleavage cytochrome P450 enzyme, cholesterol side-chain cleavage enzyme, cholesterol side-chain-cleaving enzyme, cholesterol side-cleaving enzyme, CYP 11A1, Cyp11a, CYP11A1, CYPXIA1, cytochrome P-450scc, cytochrome P450 11A1, cytochrome P450 cholesterol side chain cleavage, cytochrome P450 cholesterol side-chain cleavage, cytochrome P450 side chain cleavage enzyme, cytochrome P450-mediated cholesterol side-chain cleavage enzyme, cytochrome P450-mediated side-chain cleavage enzyme, cytochrome P450scc, desmolase, steroid 20-22, endoenzymes, cholesterol side-chain-cleaving, enzymes, cholesterol side-chain-cleaving, P450 11A1, P450 cholesterol side chain cleaving enzyme, P450 cholesterol side-chain cleavage enzyme, P450(scc), P450scc, steroid 20-22 desmolase, steroid 20-22-lyase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.15.6 cholesterol monooxygenase (side-chain-cleaving)

Cloned

Cloned on EC 1.14.15.6 - cholesterol monooxygenase (side-chain-cleaving)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
both full-length and an N-terminally truncated form amplified and inserted into TOPO TA PCR cloning vector and subsequently subcloned into the EcoRI restriction site of pCMV-tag2B vector to obtain CYP11A1 proteins with an N-terminal Flag tag or into pEGFP-C2 vector to yield an N-terminal GFP tag
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DNA and amino acid sequence determination and analysis, expression analysis and sequence comparisons
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DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, and expression in COS-1 cells with adrenodoxin resulting in significantly more pregnenolone, co-expression with the steroidogenic acute regulatory protein and a human P450scc/adrenodoxin reductase/adrenodoxin fusion constructs. COS-1 cells transfected with a modified construct in which human P450scc is replaced by Dasyatis sabina P450scc show higher rates than cells transfected with Dasyatis sabina P450scc alone. Suncloning in Escherichia coli strain DH5alpha
DNA sequence determination, quantitative enzyme expression analysis in brain prior and after treatment with 4-nonylphenol
-
expressed from pCMV-SPORT6 vector
-
expression in Escherichia coli and Saccharomyces cerevisiae
expression in the ventral tegmental area or nucleus accumbens of rat
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene CYP11A1, DNA and amino acid sequence determination and analysis, expression pattern analysis, functional expression in COS-7 cells
gene CYP11A1, DNA and amino acid sequence determination of wild-type and mutant enzymes, expression in HeLa cells and COS-1 cells, functional studies of P450scc RNA splicing
gene CYP11A1, expression as His-tagged protein in Escherichia coli
gene p450scc, semi-/quantitative RT-PCR expression analysis, overview
-
heterologous expression in Escherichia coli
-
hybrid proteins AAC-mCYP11A1, Bcs1p(1–83)-mCYP11A1, Su9(1-116)-mCYP11A1, preAd-mCYP11A1, DLD(1-72)-mCYP11A1 and COXIV-mCYP11A1 expressed in Saccharomyces cerevisiae strain 2805. Escherichia coli strain JM109 transformed with the plasmid pTrc99(A)/Ad-mCYP11A1
-
mutants and wild-type expressed in Escherichia coli
-
Nicotiana tabacum plants expressing CYP11A1 cDNA
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recombinant expression of C-terminally His4-tagged CYP11A1