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1.14.16.2: tyrosine 3-monooxygenase

This is an abbreviated version!
For detailed information about tyrosine 3-monooxygenase, go to the full flat file.

Word Map on EC 1.14.16.2

Reaction

L-tyrosine
+
a 5,6,7,8-tetrahydropteridine
 +
O2
=
L-Dopa
 +
a 4a-hydroxy-5,6,7,8-tetrahydropteridine

Synonyms

CAT-2, DTH1, DTH2, hTH2, L-tyrosine hydroxylase, monophenol monooxygenase, oxygenase, tyrosine 3-mono-, TH, TH1, TH2, TyrH, tyrosinase, tyrosine 3-hydroxylase, tyrosine 3-monooxygenase, tyrosine hydroxylase, tyrosine hydroxylase type 1, tyrosine-3-mono-oxygenase, tyrosine-3-monooxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.16 With reduced pteridine as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.16.2 tyrosine 3-monooxygenase

Storage Stability

Storage Stability on EC 1.14.16.2 - tyrosine 3-monooxygenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-30°C, immobilized and lyophilized enzyme, stored in dry environment, unaltered more than 150 days
-
-70°C, in presence of diisopropylfluorophosphate, 2 months
-
-80°C, 0.05% Tween 80, 0.1 mM EDTA, 25% glycerol, 5 mM sodium phosphate buffer, pH 7.5, 3 months without loss of activity
-
-80°C, 0.05% Tween 80, 1 mM EDTA, 25% glycerol, 3 months
-
-80°C, 20 mM Tris-HCl, pH 7.4, 8% sucrose, dithiothreitol, several months
-
-80°C, highly purified enzyme, 2 months
-
-80°C, protein concentration above 0.05 mg/ml, 2 months
-
-80°C, purified wild-type enzyme can be stored indefinetely in absence of glycerol. the mutant enzymes T245P and T463M behave similarly. The removal of glyceryl from the T283M and R306H enzymes results in significant precipitation and loss of the protein
-
4°C, 24 h, 0.05% Tween 80, 1 mM EDTA, 25% glycerol, 68% loss of activity
-
4°C, 5 h, without stabilizing agents, almost complete loss of activity
-
4°C, in presence of diisopropylfluorophosphate, 1 week
-