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1.14.16.4: tryptophan 5-monooxygenase

This is an abbreviated version!
For detailed information about tryptophan 5-monooxygenase, go to the full flat file.

Word Map on EC 1.14.16.4

Reaction

L-tryptophan
+
a 5,6,7,8-tetrahydropteridine
 +
O2
=
5-hydroxy-L-tryptophan
 +
a 4a-hydroxy-5,6,7,8-tetrahydropteridine

Synonyms

hTPH2, indoleacetic acid-5-hydroxylase, L-tryptophan 5-hydroxylase, L-tryptophan hydroxylase, oxygenase, tryptophan 5-mono-, peripheral tryptophan hydroxylase, PTMO, putative tryptophan monooxygenase, TH2, TPH, TPH-1, TPH-2, TPH1, TPH2, TRpOH, tryptophan 5-hydroxylase, tryptophan 5-monooxygenase, tryptophan hydroxylase, tryptophan hydroxylase 1, tryptophan hydroxylase 2, tryptophan hydroxylase I, tryptophan hydroxylase isoform 1, tryptophan hydroxylase isoform 2, tryptophan hydroxylase isoform2, tryptophan hydroxylase type I, tryptophan hydroxylase-1, tryptophan hydroxylase-2, tryptophan-5-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.16 With reduced pteridine as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.16.4 tryptophan 5-monooxygenase

Storage Stability

Storage Stability on EC 1.14.16.4 - tryptophan 5-monooxygenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 10% glycerol, 0.05 mM EDTA, 0.06% Tween 20, 28 d, 69% loss of activity
-
-20°C, 2 months, 40% loss of activity
-80°C, 1 month, 20% loss of activity
-
-80°C, 1 month, 30% loss of activity
-
-80°C, N2 atmosphere, dithiothreitol, 2 weeks, 20% loss of activity
-
0°C, 20 h, 60% loss of activity
-
4°C, 0.06% Tween 20, 0.05 mM EDTA, 50% ethylene glycol, 1 M NaCl, 5 days, no loss of activity
-
4°C, 10% glycerol, 0.05 mM EDTA, 0.06% Tween 20, 28 d, 34% loss of activity
-
4°C, under N2, 2 months, no loss of activity
-
4°C, under O2, 48 h, 90% loss of activity
-
N- and C-terminal truncated recombinant enzyme, -70°C, 100 mM ammonium sulfate, ferrous iron, 10% glycerol, 2 mM dithiothreitiol, pH 7.0, indefinitely, no loss of activity
-
recombinant His-tagged enzyme, -80°C, 50 mM HEPES, pH 7.5, 200 mM NaCl, 10% glycerol, 0.05% Tween 20, 1 mM dithiothreitol, 4 days, no loss of activity
recombinant His-tagged enzyme, 4°C, 50 mM HEPES, pH 7.5, 200 mM NaCl, 10% glycerol, 0.05% Tween 20, 1 mM dithiothreitol, 4 days, no loss of activity