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1.14.17.1: dopamine beta-monooxygenase

This is an abbreviated version!
For detailed information about dopamine beta-monooxygenase, go to the full flat file.

Word Map on EC 1.14.17.1

Reaction

dopamine
+ 2 ascorbate +
O2
=
noradrenaline
 + 2 monodehydroascorbate +
H2O

Synonyms

3,4-dihydroxy-phenylethylamine, ascorbate: oxygen oxidoreductase (3-hydroxylating), 3,4-dihydroxyphenethylamine beta-oxidase, 3,4-dihydroxyphenylethylamine beta-hydoxylase, 4-(2-aminoethyl)pyrocatechol beta-oxidase, DbetaH, DbetaM, DBH, DBM, Dopa beta-hydroxylase, dopamine beta hydroxylase, dopamine beta-hydrolase, dopamine beta-hydroxylase, dopamine beta-mono-oxygenase, dopamine beta-monooxygenase, dopamine beta-oxidase, dopamine hydroxylase, dopamine(3,4-dihydroxyphenethylamine)beta-mono-oxygenase, dopamine-B-hydroxylase, dopamine-beta hydroxylase, dopamine-beta-hydroxylase, dopamine-beta-monooxygenase, EC 1.14.2.1, gDBH, LvDBH, MDBH, oxygenase, dopamine beta-mono-, pDbetaH, phenylamine beta-hydroxylase, plasma DbetaH activity, plasma dopamine beta-hydroxylase, plDbetaH, SDBH, TBetaM, tyramine beta-monooxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.17 With reduced ascorbate as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.17.1 dopamine beta-monooxygenase

Molecular Weight

Molecular Weight on EC 1.14.17.1 - dopamine beta-monooxygenase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
220000 - 250000
-
220000 Da, L208 homoenzyme, 230000 Da, F208 homoenzyme, 250000 Da, F208/L208 heteroenzyme, low-angle laser light scattering-photometry
260000
-
native enzyme, low-angle laser light scattering photometry coupled with high-performance gel filtration
280000
-
SDS-PAGE with 0.01% SDS
290000
300000
316000
-
gel filtration
370000
-
in 5% ammonium sulfate buffer
450000
-
in 0.2 M NaCl
600000
-
in 1 M NaCl
610000
-
tetrameric form, SDS-PAGE
65000
-
4 * 65000, deduced from cDNA
68480
x * 68480, predicted
72000
75000
80000
-
4 * 80000, SDS-PAGE after treatment with 2-mercaptoethanol, subunits joined in pairs by disulfide bonds
800000
-
in 5 mM sodium phosphate buffer, pH 7.0
88000
-
4 * 88000 SDS-PAGE
additional information
-
molecular forms change depending on concentration of salts and kind of salts