1.14.17.3: peptidylglycine monooxygenase

This is an abbreviated version!
For detailed information about peptidylglycine monooxygenase, go to the full flat file.

Reaction

Synonyms

alpha-AE, bifunctional PAM, bifunctional peptidylglycine alpha-amidating monooxygenase, CG3832, hPHMcc, More, PAM, PAM-1, PAM-2, PAM-A, PAM-B, PAM/PHM, peptide alpha-amidating enzyme, peptide alpha-amide synthase, peptide-alpha-amide synthetase, peptidyl alpha-amidating enzyme, peptidyl alpha-hydroxylating monooxygenase, peptidyl-glycine alpha-amidating monooxygenase, peptidylglycine 2-hydroxylase, peptidylglycine alpha-amidating mono-oxygenase, peptidylglycine alpha-amidating monooxygenase, peptidylglycine alpha-hydroxylase, peptidylglycine alpha-hydroxylating monooxygenase, peptidylglycine alpha-hydroxylating-monooxygenase, peptidylglycine alpha-monooxygenase, peptidylglycine monooxygenase, peptidylglycine-alpha-amidating monooxygenase, PHM, PHMcc, synthase, peptide alpha-amide, type A PAM

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.17 With reduced ascorbate as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.17.3 peptidylglycine monooxygenase

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Results	in table
6	Activating Compound
644	AA Sequence
9	Application
1	CAS Registry Number
43	Cloned(Commentary)
70	Cofactor
2	Crystallization (Commentary)
24	Disease/ Diagnostics
1	Expression
24	General Information
4	General Stability
24	IC50 Value
162	Inhibitors
1	kcat/KM [mM/s]
79	Ki Value [mM]
168	KM Value [mM]
43	Localization
81	Metals/Ions
37	Molecular Weight [Da]
83	Natural Substrates/ Products (Substrates)
101	Organism
3	Oxidation Stability
31	PDB ID
48	pH Optimum
5	pH Range
1	pH Stability
2	Posttranslational Modification
47	Protein Variants
44	Purification (Commentary)
30	Reaction
29	Reaction Type
99	Reference
116	Source Tissue
52	Specific Activity [micromol/min/mg]
372	Substrates and Products (Substrate)
19	Subunits
193	Synonyms
1	Systematic Name
31	Temperature Optimum [°C]
1	Temperature Stability [°C]
30	Turnover Number [1/s]

Purification

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Purification on EC 1.14.17.3 - peptidylglycine monooxygenase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

8 entries

cation-exchange chromatography, anion-exchange chromatography, gel filtration

Xenopus laevis

-

716946

cell extracts are prepared

Homo sapiens

-

699036

forms A and B differing in MW and charge

Bos taurus

-

438570

large scale purification of the recombinant truncated enzyme, comprising the catalytic core residues 42-356, from CHO DG44 cells, reconstitution of copper bound to the enzyme

Rattus norvegicus

-

676976

membrane bound form

Sus scrofa

-

714748

membrane-bound form from atrial gland

Aplysia californica

-

438586

mutant H172A of type A alpha-AE, recombinant from chinese hamster ovary cells

Rattus norvegicus

-

438595

native wild-type and recombinant from Spodoptera frugiperda cell expression system

Xenopus laevis

-

438569, 438590

partially

Rattus norvegicus

-

438562

peptidylglycine alpha-hydroxylating monooxygenase and a peptidyl-alpha-hydroxyglycine alpha-amidating lyase

Bos taurus

-

438567

Poros 20 m HQ anion exchange resin column chromatography

Homo sapiens

-

726958

recombinant enzyme from CHO cell culture medium by copper-chelating affinity chromatography

Xenopus laevis

-

660417

recombinant from mouse cells

Rattus norvegicus

-

438566, 438571

recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 by glutathione affinity chromatography, tag cleavage by HRV3C protease overnight, and followed by anion echange chromatography

Mus musculus

P97467

745296

recombinant PAM from CHO dhfr cells by ammonium sulfate fractionation and anion exchange chromatography

Homo sapiens

P19021

746370

recombinant soluble enzyme from culture medium in a 3-step chromatographic procedure, about 40fold to near homogeneity

Homo sapiens

P19021

648703

recombinant wild-type and mutant enzymes

Rattus norvegicus

P14925

744361

recombinant wild-type and mutant enzymes from CHO cells by a 3-step chromatographic method

Rattus norvegicus

-

658004

recombinant wild-type and mutant enzymes from CHO DG44 cells. Copper reconstitution of recombinant purified wild-type and mutant enzymes

Rattus norvegicus

P14925

744349

thioredoxin-hPHMcc-His6 fusion is purified to homogeneity

Homo sapiens

P19021

720977

truncated mutant form 2 from gene AE-II, recombinant from Spodoptera frugiperda cell expression system

Xenopus laevis

-

438584

two chromatography steps

Rattus norvegicus

-

672059

two chromatoography steps

Rattus norvegicus

-

674739, 676976

type A alpha-AE, recombinant from chinese hamster ovary cells

2 entries

type A and B

Rattus norvegicus

-

438571

-

Cricetulus griseus

-

672113

-

Rattus norvegicus

-

438574, 715272

-

Sus scrofa

-

438563, 438579

-

Bos taurus

-

438592, 673624

-

Xenopus laevis

-

438573, 438576

-

Equus caballus

-

438580

-

Blattella germanica

-

696707

-

Rattus norvegicus

P14925

696707, 698481

type A alpha-AE, recombinant from chinese hamster ovary cells

Rattus norvegicus

-

438589, 438593, 438594

type A alpha-AE, recombinant from chinese hamster ovary cells

Rattus norvegicus

P14925

438591