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1.14.19.25: acyl-lipid omega-3 desaturase (cytochrome b5)

This is an abbreviated version!
For detailed information about acyl-lipid omega-3 desaturase (cytochrome b5), go to the full flat file.

Word Map on EC 1.14.19.25

Reaction

a linoleoyl-[glycerolipid]
+ 2 ferrocytochrome b5 +
O2
 + 2 H+ =
an alpha-linolenoyl-[glycerolipid]
 + 2 ferricytochrome b5 + 2 H2O

Synonyms

BjFad3, DELTA15 desaturase, endoplasmic reticulum-type omega-3 fatty acid desaturase, ER-localized omega-3 FA desaturase, ER-type desaturase, ER-type omega-3 fatty acid desaturase, FAD3, FAD3-1, FAD3-2, FAD3A, FAD3B, FAD3C, fatty acid desaturase 3, LeFAD3, maw3, msdd17, omega-3 desaturase, omega-3 FA desaturase, omega-3 FAD, omega-3 fatty acid desaturase, omega3-desaturase, PfrFAD3, sdd17

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
                1.14.19.25 acyl-lipid omega-3 desaturase (cytochrome b5)

Cloned

Cloned on EC 1.14.19.25 - acyl-lipid omega-3 desaturase (cytochrome b5)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae
-
expressed in Saccharomyces cerevisiae strain InvSc1
-
expressed in Saccharomyces cerevisiae strain K601
expressed in Saccharomyces verevisiae strain InvSc1
-
expression in Saccharomyces cerevisiae
gene fad3, DNA and amino acid sequence determination and analysis
gene fad3, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant plamid pCAM::Nap-BjFad3-NOS is introduced into Agrobacterium tumefaciens strain LBA4404/VirGN54D by freeze-thaw method and then used for transformation of Escherichia coli strain DH10B and of Nicotiana tabacum explant, recombinant ectopic expression analysis in leaves of transgenic plants, recombinant expression of the enzyme in transgenic Oryza sativa plants, semi-quantitative RT-PCR expression analysis
gene fad3, overexpression of sense and antisense FAD3 sequences under control of the CaMV 35S promoter, quantitative reverse transcription-PCR enzyme expression analysis, LeFAD3 overexpression enhances the tolerance of early seedlings to salinity stress. Reduced oxidative damage in LeFAD3 overexpression tomato plants under salt stress, with altered ratio of 18:3/18:2 compared to wild-type. Change in leaf cell ultrastructure under salt stress in wild-type and mutant leaves
-
gene fad3, recombinant expression of a fad3-LUC fusion construct in Arabidopsis thaliana plants through trabsfection with Agrobacterium tumefaciens strain C58 pGV3850
gene fad3-1, DNA and amino acid sequence determination and analysis, single gene, transcriptome analysis, sequence comparisons of fad genes, phylogenetic analysis, quantitative real-time and RT-PCR expression analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene fad3-2, DNA and amino acid sequence determination and analysis, single gene, transcriptome analysis, sequence comparisons of fad genes, phylogenetic analysis, quantitative real-time and RT-PCR expression analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene omega-3 FAD, genetic structure with endogenous gene and native promoter, recombinant expression of the disrupted omega-3 FAD gene in Chlorella vulgaris via Agrobacterium-mediated transformation, real-time PCR and RT-PCR enzyme expression analysis
gene sdd17, recombinant exression of codon-otimized gene in human VCaP prostate cancer cells by lentiviral tranfection using the the PLJM1 lentivirus vector, which allows the endogenous production of OMEGA-3 polyunsaturated fatty acids (PUFAs), quantitative RT-PCR expression analysis
isolation of a gene and functionally complementation of a mutant of Arabidopsis deficient in omega-3 unsaturated fatty acids