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1.14.19.29: sphingolipid 8-(E/Z)-desaturase

This is an abbreviated version!
For detailed information about sphingolipid 8-(E/Z)-desaturase, go to the full flat file.

Reaction

a (4R)-4-hydroxysphinganine ceramide
+ 2 ferrocytochrome b5 +
O2
+ 2 H+ =
a (4R,8E)-4-hydroxysphing-8-enine ceramide
+ 2 ferricytochrome b5 + 2 H2O

Synonyms

8 fatty acid desaturase, 8-sphingolipid desaturase, D8A, D8B, D8C, D8D, DELTA8 Des, Delta8 sphingolipid desaturase, DELTA8(E,Z)-sphingolipid desaturase, Delta8-fatty-acid desaturase 1, Delta8-fatty-acid desaturase 2, DELTA8-sphingolipid desaturase, Delta8-sphingolipid desaturase 1, Delta8-sphingolipid desaturase 2, Delta8-sphingolipid desaturase 3, DELTA8-sphingolipid-desaturase, DES8, DXDES, LCB DELTA8 desaturase, PtD8A, PtD8B, PtD8C, SLD, SLD1, SLD2, sphingoid long-chain base DELTA8 desaturase, sphingolipid DELTA8-desaturase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
                1.14.19.29 sphingolipid 8-(E/Z)-desaturase

Engineering

Engineering on EC 1.14.19.29 - sphingolipid 8-(E/Z)-desaturase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D208R
inactive
D210R
inactive
F59R
the mutant shows severely reduced activity compared to the wild type enzyme
G28R
the mutant shows reduced activity compared to the wild type enzyme
G368R
inactive
G52R
the mutant shows wild type activity
H63R
inactive
L369A
the mutant shows severely reduced activity compared to the wild type enzyme
L71R
the mutant shows wild type activity
N203R
inactive
Q372H
the mutant shows severely reduced activity compared to the wild type enzyme
T56R
the mutant shows reduced activity compared to the wild type enzyme
W190A
the mutant shows severely reduced activity compared to the wild type enzyme
W345A
the mutant shows severely reduced activity compared to the wild type enzyme
Y31A
the mutant shows reduced activity compared to the wild type enzyme