1.14.19.31: acyl-lipid (7-3)-desaturase

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For detailed information about acyl-lipid (7-3)-desaturase, go to the full flat file.

Word Map on EC 1.14.19.31

1.14.19.31

desaturation

docosahexaenoic

polyunsaturated

pavlova

microalgae

eicosapentaenoic

delta5

front-end

desaturases

Reaction

a (7Z,10Z,13Z,16Z)-docosa-7,10,13,16-tetraenoyl-[glycerolipid]

+ 2 ferrocytochrome b5 +

+ 2 H+ =

a (4Z,7Z,10Z,13Z,16Z)-docosa-4,7,10,13,16-pentaenoyl-[glycerolipid]

+ 2 ferricytochrome b5 + 2 H2O

Synonyms

CrDELTA4FAD, DELTA4 desaturase, DELTA4 fatty acid desaturase, DELTA4-desaturase, DELTA4-fatty acid desaturase, DELTA4Fad, Egd4p, FADS2, MGDG-specific DELTA4-desaturase, monogalactosyldiacylglycerol-specific DELTA4-desaturase, Old4p, PlDES1, PsD4Des

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.19 With oxidation of a pair of donors resulting in the reduction of O₂ to two molecules of water

1.14.19.31 acyl-lipid (7-3)-desaturase

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Results	in table
13	AA Sequence
12	Cloned(Commentary)
7	Cofactor
6	Expression
26	General Information
14	Localization
2	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
23	Organism
1	Pathway
1	Purification (Commentary)
8	Reaction
23	Reference
6	Source Tissue
33	Substrates and Products (Substrate)
4	Subunits
35	Synonyms
1	Systematic Name

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Cloned on EC 1.14.19.31 - acyl-lipid (7-3)-desaturase

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DNA and amino acid sequence determination and analysis

2 entries

DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, recombinant functional expression in Saccharomyces cerevisiae strain HH3

3 entries

expression in MCF-7 human breast cancer cells. MCF-7 cells stably expressing FADS1 and FADS2

Papio anubis

B8R1K0

744864

expression in yeast and in Arabidopsis

Rebecca salina

A0PJ29

732616

gene CrDELTA4FAD, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GFP-tagged enzyme in Chlamydomonas reinhardtii chloroplasts, recombinant expression of the His-tagged cytochrome b5 domain (resdues 48-147) in Escherichia coli strain BL21(DE3)

Chlamydomonas reinhardtii

I2CYZ4

733753

gene Egd4, DNA and amino acid sequence determination and analysis, phylogenetic tree, partly codon-optimized version of the cDNA, sequence comparison, recombinant expression in Saccharomyces cerevisiae strain INVSc1 in endoplasmic reticulum membrane,fatty acid analysis, overview. Expression of GFP-tagged enzyme

Euglena gracilis

Q6WNG7

733779

gene Old4, DNA and amino acid sequence determination and analysis, phylogenetic tree, partly codon-optimized version of the cDNA, sequence comparison, recombinant expression in Saccharomyces cerevisiae strain INVSc1 in endoplasmic reticulum membrane,fatty acid analysis, overview. Expression of GFP-tagged enzyme, GFP-Old4p does not form aggregates in yeast cells and localizes to the endoplasmic reticulum

Ostreococcus sp. 'lucimarinus'

A4RQJ7

733779

gene TpdesK, encoded on chromosome 5, the gene has a single intron, phylogenetic analysis and tree, recombinant expression in Saccharomyces cerevisiae strain W303A1

Thalassiosira pseudonana

658684

heterologous expression in Saccharomyces cerevisiae and Brassica juncea respectively. Introduction of the full-length enzyme into Brassica juncea under the control of the 35S promoter results in the production of (4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaeno in leaves when exogenously supplied with (7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoic acid

Thraustochytrium sp.

Q8S3C0

732026