1.14.19.34: acyl-lipid (9+3)-(E)-desaturase

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For detailed information about acyl-lipid (9+3)-(E)-desaturase, go to the full flat file.

Reaction

Synonyms

acyl-lipid 12-(E)-desaturase, DELTA12 oleate desaturase, DELTA12 oleic acid desaturase, DELTA12-oleic acid desaturase, DsFAD2-1, DsFAD2-2, FAD2, FADX

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.19 With oxidation of a pair of donors resulting in the reduction of O₂ to two molecules of water

                1.14.19.34 acyl-lipid (9+3)-(E)-desaturase

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6	AA Sequence
4	Cloned(Commentary)
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6	General Information
1	Localization
1	Metals/Ions
4	Natural Substrates/ Products (Substrates)
4	Organism
1	Pathway
3	Reaction
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6	Substrates and Products (Substrate)
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Cloned on EC 1.14.19.34 - acyl-lipid (9+3)-(E)-desaturase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

gene DsFAD2-1, DNA and amino acid sequence determination and analysis, recombinant expression of DsFAD2-1 in Saccharomyces cerevisiae strain YPH499 and Glycine max somatic embryos resulting in the accumulation of the trans-DELTA12 isomer of linoleic acid (18:2
9cis,12trans) rather than the more typical cis-DELTA12 isomer. When co-expressed with DsFAD2-1 in soybean embryos or yeast, DsFAD2-2 converts 18:2
9cis,12trans into dimorphecolic acid

Dimorphotheca sinuata

Q6RS95, Q6RS96

734145

gene DsFAD2-2, DNA and amino acid sequence determination and analysis, recombinant expression of DsFAD2-2 in Saccharomyces cerevisiae strain YPH499 and Glycine max somatic embryos. When co-expressed with DsFAD2-1 in soybean embryos or yeast, DsFAD2-2 converts 18:2
9cis,12trans into dimorphecolic acid. When DsFAD2-2 is expressed alone in soybean embryos or together with a typical cis-DELTA12-oleic acid desaturase in yeast, trace amounts of the cis-DELTA12 isomer of dimorphecolic acid (9-OH-18:2DELTA9cis,12cis) are formed from DsFAD2-2 activity with cis-DELTA12-linoleic acid

Dimorphotheca sinuata

Q6RS95, Q6RS96

734145

gene FAD2, combined transgenic expression of Punica granatum conjugase (FADX, EC 1.14.19.33) under the control of a seed-specific napin promoter and Arabidopsis thaliana FAD2 desaturase in high linoleic acid Arabidopsis thaliana fad3/fae1 mutant, via Agrobacterium tumefaciens cell strain GV3101 transformation, leads to increased accumulation of punicic acid, quantitative RT-PCR enzyme expression analysis

Punica granatum

Q84VT2

734991

gene FAD2, DNA and amino acid sequence determination and analysis, functional expression of the enzyme in Saccharomyces cerevisiae strain MMYO11,
transient expression of myc-tagged enzyme in Nicotiana tabacum cv. Bright-Yellow 2 suspension-cultured BY-2 cells

Vernicia fordii

Q8GZC3

732650