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1.14.19.35: sn-2 acyl-lipid omega-3 desaturase (ferredoxin)

This is an abbreviated version!
For detailed information about sn-2 acyl-lipid omega-3 desaturase (ferredoxin), go to the full flat file.

Word Map on EC 1.14.19.35

Reaction

a (7Z,10Z)-hexadeca-7,10-dienoyl-[glycerolipid]
+ 2 reduced ferredoxin +
O2
 + 2 H+ =
a (7Z,10Z,13Z)-hexadeca-7,10,13-trienoyl-[glycerolipid]
 + 2 oxidized ferredoxin + 2 H2O

Synonyms

16:2/18:2 desaturase, acyl-lipid omega-3 desaturase, FAD7, FAD7 omega 3-desaturase, FAD7-1, FAD7-2, FAD8, fatty acid desaturase 7, fatty acid desaturase-7, fatty acid desaturase7, GmFAD7, HaFAD7, HvFAD7, omega-3 desaturase, omega-3 FA desaturase, omega-3 fatty acid desaturase, omega-3 fatty acid desaturase gene, omega3 fatty acid desaturase, omega3-fatty acid desaturase, OsFAD8, PfrFAD7-1, PfrFAD7-2, plastid omega-3 desaturase, plastidial FAD7 omega-3 desaturase, plastidial omega-3 desaturase, plastidial omega-3 fatty acid desaturase, Sefad7, sn-2 acyl-lipid omega-3 desaturase (ferredoxin)

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
                1.14.19.35 sn-2 acyl-lipid omega-3 desaturase (ferredoxin)

Cloned

Cloned on EC 1.14.19.35 - sn-2 acyl-lipid omega-3 desaturase (ferredoxin)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene fad7, construction of cDNA and genomic DNA libraries, and screening using Arabidopsis thaliana gene fad7 as template, the genomic clone has 8 exons, DNA and amino acid sequence determination and analysis, genomic organization, phylogenetic analysis
gene fad7, DNA and amino acid sequence determination and analysis
gene fad7, DNA and amino acid sequence determination and analysis using gene fad3 as template. The fad7 and fad8 gene products are functionally equivalent, although the two genes share only about 75% nucleotide identity
gene FAD7, DNA and amino acid sequence determination and analysis, genotyping of wild-type and mutants
gene FAD7, DNA and amino acid sequence determination and analysis, genotyping, expression of T286 enzyme mutants
gene fad7, DNA and amino acid sequence determination and analysis, sequence comparisons,quantitative real-time PCR enzyme expression analysis, phylogenetic analysis
gene fad7, enzyme expression analysis, recombinant expression of fad7 in wild-type Rschew ecotype WF11 cell line and enzyme-deficient mutant cell line SH10 derived from JB101/Col-0
gene FAD7, expression of the -825 Arabidopsis FAD7 promoter-P-glucuronidase fusion gene in Nicotiana tabacum cv. W38
gene fad7, expression of wild-type and chimeric mutant enzymes with or without a c-Myc epitope tag in transgenic Arabidopsis thaliana plants
gene fad7, gene expression profiles, overview. The FAD7 gene expression is differentially regulated in tomato varieties Gerry and T47657 under the effect of cold stress in the short and long term
gene fad7, recombinant expression in Nicotiana tabacum cv. SR1 leaves, that contain increased levels of 16:3 and 18:3 fatty acids, and correspondingly decreased levels of their precursors, hexadecadienoic and linoleic acids, via using Agrobacterium tumefaciens C58ClRif (pGV2280) containing the plasmid pTiDES7 or p501-17. The expression of FAD7 leads to reduced suppression of leaf growth at 1°C compared to wild-type plants. The low-temperature-induced chlorosis is also much reduced in the plants transformed with the fad7 gene. Control growth at 25°C
gene fad7, sequence comparison with FAD3, recombinant expression in Saccharomyces cerevisiae strain W303-1A, plastidial omega3-fatty acid desaturase contains the signalling determinants required for targeting to, and retention in, the endoplasmic reticulum membrane in Saccharomyces cerevisiae but requires co-expressed ferredoxin for activity. Heterologous enzyme expression in yeast shows low activity in contrast to similar expression of microsomal FAD3 omega 3-desaturases. Expression of enzyme variants N-terminally tagged with HA, and/or anchor domains ELO or FAE, and containing a KKNL motif at the C-terminus. Deletion of the endogenous KEK C-terminal motif results in dramatically reduced accumulation of FAD7-HA. Coexpression of ferredoxin
gene fad7, sequence comparisons, recombinant functional expression in Nicotiana tabacum cv. Jayanti seeds and leaves targeted to the endoplasmic reticulum, using the Agrobacterium tumefaciens strain EHA105 transfection method and the binary vector pCAMBIA1300 with CaMV35S promoter, quantitative real-time PCR expression analysis
gene fad7, the promoter sequence of gene fad7 contains several short sequence cis-elements and the -825 bp regulatory region, analysis, overview
gene fad7, two splicing variants fad7-1 and fad7-2, DNA and amino acid sequence determination and analysis, single gene, transcriptome analysis, sequence comparisons of fad genes, phylogenetic analysis, quantitative real-time and RT-PCR expression analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1, where pYES2-PfrFAD7-1 transformants produce 18:3 when supplemented with 18:2
gene fad7, two splicing variants fad7-1 and fad7-2, DNA and amino acid sequence determination and analysis, single gene, transcriptome analysis, sequence comparisons of fad genes, phylogenetic analysis, quantitative real-time and RT-PCR expression analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1, where pYES2-PfrFAD7-2 transformants produce 18:3 when supplemented with 18:2
gene fad8, construction of cDNA and genomic DNA libraries, and screening using Arabidopsis thaliana gene fad7 as template, the genomic clone has 7 exons, DNA and amino acid sequence determination and analysis, genomic organization, phylogenetic analysis
gene fad8, DNA and amino acid sequence determination and analysis
gene fad8, DNA and amino acid sequence determination and analysis using gene fad3 as template, constitutive expression of the fad8 cDNA in transgenic plants of a fad7 mutant JBl01, via Agrobacterium turnefaciens C58 (pGV3101) containing plasmid pBI/fad8 or pBIl2l, results in genetic complementation of the mutation, indicating that the fad7 and fad8 gene products are functionally equivalent. Expression of the fad8 cDNA in transgenic plants often results in the co-suppression of both the endogenous fad7 and fad8 genes in spite of the fact that these two genes share only about 75% nucleotide identity
gene FAD8, DNA and amino acid sequence determination and analysis, genotyping of wild-type and mutants. Recombinant expression of AtFAD8-YFP fusion protein in stable transgenic Arabidopsis thaliana lines and transiently in Nicotinana benthamiana leaves, overexpression of YFP-tagged fad8 in Arabidosis thaliana results in increased 18:3 trienoic fatty acid levels in phospholipids but not in galactolipids
gene fad8, DNA and amino acid sequence determination and analysis, sequence comparisons,quantitative real-time PCR enzyme expression analysis, phylogenetic analysis
gene fad8, expression of wild-type and chimeric mutant enzymes with or without a c-Myc epitope tag in transgenic Arabidopsis thaliana plants
gene fad8, recombinant expression in Nicotiana tabacum BY-2 cells and in transgenic tobacco plants via Agrobacterium-mediated transformation. Co-expression of Arabidopsis thaliana FAD8 and Brassica napus FAD3, EC 1.14.19.25, under the control of the 35S CaMV promoter leads to increase in 18:3, which is compensated mainly by the decrease in 18:2, but is also accompanied by a decrease in oleic acid (18:1). Additionally, there is a small but significant decrease in stearic acid (18:0) which seems to be compensated by an increase in palmitic acid (16:0). In the BY-2-FAD8 cells, the ratio of 18:3 to 18:2 is about twofold higher than that in the BY-2 cells. Fatty acid composition of leaf polar lipids of wild-type and transgenic plants, overview
gene OsFAD8, located on chromosome 7, DNA and amino acid sequence determination and analysis
heterologous expression in Synechocystis sp. PCC 6803