1.14.19.36: sn-1 acyl-lipid omega-3 desaturase (ferredoxin)
This is an abbreviated version!
For detailed information about sn-1 acyl-lipid omega-3 desaturase (ferredoxin), go to the full flat file.
Word Map on EC 1.14.19.36
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1.14.19.36
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desaturases
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desaturation
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low-temperature
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trienoic
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soybean
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synechocystis
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alpha-linolenic
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delta12
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perception
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cyanobacterium
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eicosapentaenoic
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acclimation
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gamma-linolenic
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arachidonic
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unsaturated
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polyunsaturated
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photoautotrophic
- 1.14.19.36
-
desaturases
-
desaturation
-
low-temperature
-
trienoic
- soybean
- synechocystis
-
alpha-linolenic
-
delta12
-
perception
- cyanobacterium
-
eicosapentaenoic
-
acclimation
-
gamma-linolenic
-
arachidonic
- unsaturated
-
polyunsaturated
-
photoautotrophic
Reaction
+ 2 reduced ferredoxin + + 2 H+ = + 2 oxidized ferredoxin + 2 H2O
Synonyms
DELTA15 desaturase, DesB, DesB protein, FA omega3-desaturase, FAD8, More, Omega 3 acyl-lipid desaturase, omega3 desaturase, omega3 fatty acid desaturase, omega3-FA-desaturase, plastidial FAD8 omega-3 desaturase, plastidial omega-3 desaturase, terminal omega3-desaturase
ECTree
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Engineering
Engineering on EC 1.14.19.36 - sn-1 acyl-lipid omega-3 desaturase (ferredoxin)
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additional information
construction of a insertional mutant fad8i (SALK_093590) inactivation mutant of FAD8, analysis of the fatty acid composition of the fad8i mutant shows compensatory responses at the trienoic fatty acid level as a result of inactivation of the FAD8 gene. Genomic analysis of fad8i plants show insertion of an inverted tandem T-DNA 133 bp downstream of the start codon, within exon 1 of the AtFAD8 gene, absence of AtFAD8 transcript in the mutant. Contruction of a fad7/fad8 double knockout mutant. Given the strong reduction (70%) in 18:3 content observed in the fad7/fad8 double mutant, in mutant fad7i after disruption of the AtFAD7 gene, FAD8 enzymatic activity is able to maintain, at least partially (43.7%), the amount of 18:3 and to a much lesser extent that of 16:3 (23.2%) at 22°C. Mutant genotypes and phenotypes, overview. Temperature-dependent expression levels. Disruption of the AtFAD8 gene in the insertion mutant does not result in significant changes in the transcript levels of FAD8. Overexpression of FAD8 increased 18:3 levels in phospholipids but not in galactolipids
additional information
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construction of a insertional mutant fad8i (SALK_093590) inactivation mutant of FAD8, analysis of the fatty acid composition of the fad8i mutant shows compensatory responses at the trienoic fatty acid level as a result of inactivation of the FAD8 gene. Genomic analysis of fad8i plants show insertion of an inverted tandem T-DNA 133 bp downstream of the start codon, within exon 1 of the AtFAD8 gene, absence of AtFAD8 transcript in the mutant. Contruction of a fad7/fad8 double knockout mutant. Given the strong reduction (70%) in 18:3 content observed in the fad7/fad8 double mutant, in mutant fad7i after disruption of the AtFAD7 gene, FAD8 enzymatic activity is able to maintain, at least partially (43.7%), the amount of 18:3 and to a much lesser extent that of 16:3 (23.2%) at 22°C. Mutant genotypes and phenotypes, overview. Temperature-dependent expression levels. Disruption of the AtFAD8 gene in the insertion mutant does not result in significant changes in the transcript levels of FAD8. Overexpression of FAD8 increased 18:3 levels in phospholipids but not in galactolipids
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additional information
the desB gene in Synechocystis sp. PCC 6803 is mutated by insertion of a kanamycin-resistance gene cartridge. The resultant mutant is unable to desaturate fatty acids at the omega3 position