1.14.20.1: deacetoxycephalosporin-C synthase
This is an abbreviated version!
For detailed information about deacetoxycephalosporin-C synthase, go to the full flat file.
Word Map on EC 1.14.20.1
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1.14.20.1
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clavuligerus
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chrysogenum
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acremonium
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isopenicillin
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penicillium
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beta-lactams
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epimerase
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cephamycins
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cephalosporium
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ring-expansion
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7-aminodeacetoxycephalosporanic
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pcbab
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ironii
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cephem
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lactamdurans
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synthesis
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7-adca
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2-oxoglutarate-dependent
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cephalexin
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carbenicillin
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doacs
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biotechnology
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medicine
- 1.14.20.1
- clavuligerus
- chrysogenum
- acremonium
- isopenicillin
- penicillium
- beta-lactams
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epimerase
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cephamycins
- cephalosporium
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ring-expansion
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7-aminodeacetoxycephalosporanic
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pcbab
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ironii
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cephem
- lactamdurans
- synthesis
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7-adca
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2-oxoglutarate-dependent
- cephalexin
- carbenicillin
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doacs
- biotechnology
- medicine
Reaction
Synonyms
acDAOC/DACS, cefE, cefEF, Cephalosporin biosynthesis expandase/hydroxylase, DAOC synthase, DAOC/DAC synthase, DAOC/DACS, DAOCS, deacetoxy/deacetylcephalosporin C synthase, deacetoxycephalosporin C synthase, deacetoxycephalosporin-C synthase, deacetoxycephalosporin-C synthetase, deacetoxycephalosporin/deacetylcephalosporin C synthase, expandase, expendase, penicillin N expandase, scDAOCS
ECTree
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KM Value
KM Value on EC 1.14.20.1 - deacetoxycephalosporin-C synthase
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0.79
ampicillin
pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258F
1.3
ampicillin
pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258L
1.9
ampicillin
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258F
2.6
ampicillin
pH 7.5, cosubstrate 2-oxoglutarate, recombinant wild-type enzyme
2.6
ampicillin
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258L
3.3
ampicillin
pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258Q
4.1
ampicillin
pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258H
4.2
ampicillin
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutants R258H and R258A
4.5
ampicillin
pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258A
6.6
ampicillin
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258Q
13
ampicillin
pH 7.5, cosubstrate 2-oxoglutarate, recombinant mutant R258K
15
ampicillin
pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258K
24
ampicillin
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258K
0.014
recombinant clone FF8 derived from gene shuffling
0.75
penicillin G
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pH 6.5, 30°C, recombinant mutants G79E and I305M
0.79
penicillin G
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutants R258A and R258Q
0.84
penicillin G
pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258F
1.1
penicillin G
pH 7.5, cosubstrate 2-oxoglutarate, recombinant wild-type enzyme
1.2
penicillin G
pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258L
1.5
penicillin G
pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutants R258H and R258Q
1.6
penicillin G
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258F
1.6
penicillin G
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mutant C155Y/Y184H/V275I/C281Y, pH and temperature not specified in the publication
1.62
penicillin G
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mutant I305M/S261M, pH and temperature not specified in the publication
1.7
penicillin G
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258L
1.8
penicillin G
pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258A
1.9
penicillin G
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wild-type enzyme, pH not specified in the publication, temperature not specified in the publication
1.92
penicillin G
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mutantI305M/T213V/M73T, pH and temperature not specified in the publication
2
penicillin G
pH 7.5, 30°C, recombinant mutant DELTA310/N305L/M306I
2
penicillin G
30°C, triple mutant with truncation at residue 310, M306I and N305L
2.1
penicillin G
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mutant I305L, pH and temperature not specified in the publication
2.49
penicillin G
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mutant I305M/T213V/S261M, pH and temperature not specified in the publication
3
penicillin G
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutants R258H
3.22
penicillin G
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mutant I305M/T213V, pH and temperature not specified in the publication
3.58
penicillin G
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mutant I305M, pH and temperature not specified in the publication
4.7
penicillin G
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258K
4.71
penicillin G
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recombinant mutant Y184A, pH not specified in the publication, 30°C
5.28
penicillin G
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recombinant mutant S261A, pH not specified in the publication, 30°C
6.4
penicillin G
pH 7.5, cosubstrate 2-oxoglutarate, recombinant mutant R258K
6.42
penicillin G
30°C, double mutant with truncation at residue 310 and M306I
7
penicillin G
pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258K
8.2
penicillin G
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mutant R308I, pH not specified in the publication, temperature not specified in the publication
9.9
penicillin G
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recombinant mutant T213V, pH not specified in the publication, 30°C
11
penicillin G
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mutant R308L, pH not specified in the publication, temperature not specified in the publication
14.38
penicillin G
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wild-type enzyme, pH and temperature not specified in the publication
14.38
penicillin G
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recombinant wild-type enzyme, pH not specified in the publication, 30°C
20.6
penicillin G
recombinant His-tagged enzyme, pH 7.5, 30°C
41.16
penicillin G
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recombinant mutant Q126M, pH not specified in the publication, 30°C
48.1
penicillin G
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recombinant mutant S261M, pH not specified in the publication, 30°C
0.006
Penicillin N
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pH 6.5, 30°C, recombinant mutants C281Y and I305L
0.012
Penicillin N
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pH 6.5, 30°C, recombinant mutants V275I and I305M
additional information
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KM-values for mutant enzymes with penicillin G or Ampicillin as prime substrate and 2-oxo-4-methylpentanoate, 2-oxoglutarate or 2-oxohexanoate as cosubstrate
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additional information
additional information
KM-values for mutant enzymes with penicillin G or Ampicillin as prime substrate and 2-oxo-4-methylpentanoate, 2-oxoglutarate or 2-oxohexanoate as cosubstrate
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additional information
additional information
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Km-values for mutant enzymes with penicillin N and penicillin G as substrate
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additional information
additional information
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KM-value of Fe2+ is 0.071 mM
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additional information
additional information
pre-steady-state and steady-state kinetics, rapid quench flow and stopped-flow kinetics
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