1.14.99.15: 4-methoxybenzoate monooxygenase (O-demethylating)

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For detailed information about 4-methoxybenzoate monooxygenase (O-demethylating), go to the full flat file.

Reaction

Synonyms

4-methoxybenzoate 4-monooxygenase (O-demethylating), 4-methoxybenzoate monooxygenase, 4-methoxybenzoate O-demethylase, CYP199A2, CYP199A4, cytochrome P450 199A2, oxygenase, 4-methoxybenzoate 4-mono- (O-demethylating), p-anisic O-demethylase, piperonylate-4-O-demethylase, RPA1871

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.99 Miscellaneous

                1.14.99.15 4-methoxybenzoate monooxygenase (O-demethylating)

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Results	in table
9	Activating Compound
25	AA Sequence
1	Application
1	CAS Registry Number
8	Cloned(Commentary)
26	Cofactor
3	Crystallization (Commentary)
11	General Information
6	General Stability
36	Inhibitors
37	KM Value [mM]
15	Metals/Ions
3	Molecular Weight [Da]
21	Natural Substrates/ Products (Substrates)
38	Organism
3	Oxidation Stability
2	Pathway
8	pH Optimum
1	pH Range
1	Posttranslational Modification
20	Protein Variants
8	Purification (Commentary)
4	Reaction
13	Reaction Type
21	Reference
55	Specific Activity [micromol/min/mg]
4	Storage Stability
127	Substrates and Products (Substrate)
4	Subunits
21	Synonyms
1	Systematic Name
12	Temperature Optimum [°C]
1	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 1.14.99.15 - 4-methoxybenzoate monooxygenase (O-demethylating)

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, freeze-dried partially purified reductase stable for months without loss of activity, purified reductase is 3-4 weeks stable after addition of NADH in N2-atmosphere

Pseudomonas putida

-

438344, 438350

0-4°C, more than 24 days stable in crude extract under anaerobic conditions

Pseudomonas putida

-

438344

4°C, concentrated putidamonooxin stable for several months in N2-atmosphere with DTT and dithionite

Pseudomonas putida

-

438350