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1.14.99.29: deoxyhypusine monooxygenase

This is an abbreviated version!
For detailed information about deoxyhypusine monooxygenase, go to the full flat file.

Word Map on EC 1.14.99.29

Reaction

[eIF5A]-deoxyhypusine
+
reduced acceptor
 +
O2
=
[eIF5A]-hypusine
 +
acceptor
 +
H2O

Synonyms

deoxyhypusine hydroxylase, deoxyhypusine hydroxylase homologue nero, deoxyhypusine synthase/hydroxylase, deoxyhypusyl hydroxylase, DOHH, DOOH, hDOHH, Lia1, More, nero, oxygenase, deoxyhypusine di-

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.99 Miscellaneous
                1.14.99.29 deoxyhypusine monooxygenase

Cloned

Cloned on EC 1.14.99.29 - deoxyhypusine monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
construction of a polycistronic vector encoding eIF5A, DHS and DOHH, expression in Escherichia coli strain BL21(DE3), overview. Effects of time and temperature on the expression of recombinant proteins and hypusine modification, DOHH effectively hydroxylates all the eIF5A(Dhp), a deoxyhypusine-containing intermediate, formed
-
DNA and amino acid sequence determination and analysis, different clones, sequence comparisons, expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain JM109
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)pLysS cells
expressed in Escherichia coli BL21-CodonPlus (DE3) cells
expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
for expression in Escherichia coli cells
-
gene dohh, cloned from a GST-ORF library in a biochemical genomics approach. The dohh gene complements a yeast deficient mutant YJR070C, expression as GST-tagged protein
-
gene dohh, quantitative expression analysis
gene DOHH, quantitative real-time PCR expression analysis
-
gene dohh, sequence comparison, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene LIA1, expression in Escherichia coli strain BL21(DE3) as GST-tagged protein
-
into the pST-Acceptor vector for sequencing, into the vectors pET-15b and pET-28a for expression in Escherichia coli BL21DE3 cells
single copy gene dohh
single copy gene dohh, expression as GST-tagged protein, expression of the hypusine pathway genes in Escherichia coli as His-tagged proteins
-