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1.15.1.2: superoxide reductase

This is an abbreviated version!
For detailed information about superoxide reductase, go to the full flat file.

Word Map on EC 1.15.1.2

Reaction

superoxide
+
reduced rubredoxin
 + 2 H+ =
H2O2
 +
oxidized rubredoxin

Synonyms

1Fe SOR, 1Fe-SOR, 1Fe-superoxide reductase, 2Fe-SOR, class I SOR, class I superoxide reductase, class II SOR, cytochrome c–superoxide oxidoreductase, desulfoferrodoxin, desulforedoxin, Dfx, EC 1.18.96.1, Fe-SOR, GiSOR, MM_0632, More, neelaredoxin, neelaredoxin-type SOR, Nlr, PfSOR, rubredoxin oxidoreductase, SOR, superoxide reductase, TM0658, two-iron superoxide reductase, Zn/Fe-SOR

ECTree

     1 Oxidoreductases
         1.15 Acting on superoxide as acceptor
             1.15.1 Acting on superoxide as acceptor (only sub-subclass identified to date)
                1.15.1.2 superoxide reductase

Cloned

Cloned on EC 1.15.1.2 - superoxide reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of the desulfoferrodoxin, encoded adjacent to a gene encoding a type I rubredoxin forming a single transcriptional unit
-
DNA and amino acid sequence analysis, phylogenetic tree
expressed as recombinant protein in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli strain BL21(DE3)
Megalodesulfovibrio gigas
-
expression in Escherichia coli strain BL21-Gold (DE3)
-
expression of class I Dfx, phylogenetic analysis
expression of class II Nlr and of class I Dfx, phylogenetic analysis
expression of class II Nlr, phylogenetic analysis
expression of class III enzyme in Escherichia coli, phylogenetic analysis
-
expression of Dfx in Escherichia coli strain BL21
expression of wild-type and mutant enzymes in Escherichia coli strain XL2Blue
-
expression of wild-type and mutant SORs, and of rubredoxin in Escherichia coli strain BL21(DE3)
-
gene dsr, expression of class II Nlr in Escherichia coli, phylogenetic analysis
Megalodesulfovibrio gigas
-
gene nlr, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain XL-2 Blue, expression in Escherichia coli strain BL21(DE3)
-
gene rbo, expression of class I Dfx, phylogenetic analysis
gene sor, complementation of the Escherichia coli QC2375 mutant strain
overexpression in Escherichia coli strain BL21(DE3)-Gold
-
overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
PfSOR, synthesis of an artificial DNA sequence (referred to as SOR-as) based on the PfSOR gene sequence No AE010234 and on codon bias of Bombyx mori and expression as FLAG-tagged enzyme in transgenic Bombyx mori strain A4SOR and in Bombyx mori BmE cells, the A4SOR insertion occurs on chromosome 10 in an intergenic region separated from the nearest genes to the left and right by 22 and 47 kb, respectively, Non-diapausing DZ embryos are microinjected with pb-A4SOR. Construction of vector 1180-hr3-A4P-Flag-SOR-His-SV4 and functionalrecombinant overexpression of C-terminally His-tagged enzyme, RT-PCR enzyme expression analysis and patterns in recombinant cells and transgenic in 8-day-old eggs, hatched silkworms, first instar molts, second instar larvae, second instar molts, third instar larvae, third instar molts, fourth instar larvae, fourth instar molts, fifth instar larvae, wandering silkworm, and pupae, overview
-
phylogenetic analysis
-
recombinant expression
-
the enzyme can be produced as a functional enzyme in planta and that plants producing SOR have enhanced tolerance to heat, light, and chemically induced reactive oxygen species
the enzyme is heterologously overexpressed in Escherichia coli