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1.16.1.1: mercury(II) reductase

This is an abbreviated version!
For detailed information about mercury(II) reductase, go to the full flat file.

Word Map on EC 1.16.1.1

Reaction

Hg
+
NADP+
+
H+
=
Hg2+
+
NADPH

Synonyms

bacterial mercuric reductase, Mer A, MerA, MerA protein, mercurate(II) reductase, mercuric (II) reductase, mercuric ion reductase, mercuric reductase, mercury reductase, Msed_1241, MseMerA, reduced NADP:mercuric ion oxidoreductase, reductase, mercurate(II), Rm CH34, Tn501 MerA, Tn501 mercuric ion reductase

ECTree

     1 Oxidoreductases
         1.16 Oxidizing metal ions
             1.16.1 With NAD+ or NADP+ as acceptor
                1.16.1.1 mercury(II) reductase

Cloned

Cloned on EC 1.16.1.1 - mercury(II) reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned and expressed constitutively in Escherichia coli
-
expression in Escherichia coli
gene merA
gene merA and mer operon, expression of MerA catalytic core and NmerA proteins in Escherichia coli strain XL-1 Blue
gene merA, DNA and amino acid sequence determination and analysis, phylogenetic analysis
gene merA, DNA and amino acid sequence determination and analysis, pylogenetic analysis and tree, recombinant expression in Escherichia coli strain BL21(DE3)
gene merA, expression as wild-type and mutant N-terminally His6-tagged and maltose-binding protein fusion proteins with a 3C protease cleavage site in Escherichia coli strain TOP10 and C43
-
gene merA, genetic organization, DNA and amino acid sequence determination and analysis, sequence comparisons, and phylogenetic analysis and tree
gene merA, mer operon located on Tn5041, DNA and amino acid sequence determination and analysis, chromosomal localization, real-time PCR enzyme expression analysis
-
gene merA, overexpression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene merA, recombinant expression in Escherichia coli strain CM037 harbouring the Tn4378 transposon which contains a mer operon of Rm CH34
-
gene merA, recombinant expression in transgenoc Nicotiana tabacum cv. Xanthium plants using gene transfer by Agrobacterium tumefaciens. Transgenic tobacco expressing merA volatilizes significantly more mercury than wild-type plants. Subcloning in Escherichia coli strains DH5alpha and BL21(DE3)
gene merA, the MerA protein is encoded by the mer operon on transposon Tn501, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
gene Msed_1241, phylogenetic analysis and tree, recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3)
merA, DNA and amino acid sequence determination of genes from bacteria isolated from surface and sub-surface floodplain soil, phylogenetic analysis, overview
plasmid transfer to mercury sensitive Escherichia coli strain DH5alpha, overexpression of gene merA as His-tagged protein in Escherichia coli BL21(DE3)Plys cells
-
recombinant expression in Escherichia coli strain BL21(DE3) pLysS
-
subcloning and expression in strain TG2, bacterial two hybrid assays are performed in strain BTH101
-